HISTOPATHOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) HEART TISSUE

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YÖN, Nazan Deniz ; ÖZTÜRK, Burcu ; ZENGİN, Mustafa ; AKBULUT, Cansu (2019)
  • Publisher: Engin TUTKUN
  • Journal: 2.İnternational Congress Of Forensic Toxicology (issn: 2149-4711, eissn: 2149-4711)
  • Subject: HISTOPATHOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) HEART TISSUE

Examination thehistopathological effects of (4s)-2-(4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylicacid on heart tissue of zebrafish were aimed.Introduction:(4S)-2-(4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic acid is newsynthesized substance which obtained from cysteine and valine. Because ofthiazolidine derivates have important biological responses scientist workintensively on these compounds recent years. It is obvious that thiazolidinecontained compounds will be used in future in the pharmaceutical industry totreat important diseases. After one-weekadaptation period zebrafish divided into four group (n=10) as one control and 3experimental groups (0,2 mM, 0,4 mM, 0,6 mM). For investigating the effects ofthiazolidine, heart tissues were dissected after 5 day of the exposure. Tissueswere fixed with 10% neutral buffered formalin and dehydration were carried outin an ascending series of ethanol. After tissues were cleared in xylene,embedded in paraffin wax and cut into 5 µm sections on a microtome. Thesections were stained with hematoxylin(H&E) and PAS. Results were evaluatedwith light microscope.In control groupnormal heart histology was observed. Bulb arteriosus, ventriculus, muscle celland endocardial cell were monitored clearly. In 0,2 mM exposure group,degenerated muscle cells were detected. Hemorrhage at ventriculus, hyperplasia,vacuolization and pleomorphic case at muscle cells, degeneration at pericardiumwere observed. In 0,4 mM exposure group, hyperplasia at atrium endothelialcells, vacuolization and degeneration at muscle cells were detected. In 0,6 mMexposure group, leukocyte infiltration and pleomorphic case at ventriculus,degeneration and vacuolization at pericardial cells, hyperplasia at bulbusarteriosus were monitored.
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