publication . Other literature type . Article . 2019

3D-printed Point-of-Care Platform for Genetic Testing of Infectious Diseases Directly in Human Samples Using Acoustic Sensors and a Smartphone

George Papadakis; Alexandros K. Pantazis; Maria Ntogka; Konstantinos Parasyris; Gesthimani-Ioanna Theodosi; Georgia Kaprou; Electra Gizeli;
  • Published: 09 Apr 2019
  • Publisher: American Chemical Society (ACS)
Abstract
The objective of this work is to develop a methodology and associated platform for nucleic acid detection at the point-of-care (POC) that is sensitive, user-friendly, affordable, rapid, and robust. The heart of this system is an acoustic wave sensor, based on a Surface Acoustic Wave (SAW) or Quartz Crystal Microbalance (QCM) device, which is employed for the label-free detection of isothermally amplified target DNA. Nucleic acids amplification and detection is demonstrated inside three crude human samples, i.e., whole blood, saliva, and nasal swab, spiked in with 10–100 Salmonella cells. To qualify for POC applications, a portable platform was developed based on...
Subjects
free text keywords: Peristaltic pump, Acoustics, Quartz crystal microbalance, Surface acoustic wave, Point of care, Loop-mediated isothermal amplification, Fluidics, Acoustic wave, Antenna analyzer, Materials science
Related Organizations
Funded by
EC| LoveFood2Market
Project
LoveFood2Market
A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument
  • Funder: European Commission (EC)
  • Project Code: 687681
  • Funding stream: H2020 | IA
,
EC| CATCH-U-DNA
Project
CATCH-U-DNA
Capturing non-Amplified Tumor Circulating DNA with Ultrasound Hydrodynamics
  • Funder: European Commission (EC)
  • Project Code: 737212
  • Funding stream: H2020 | RIA
Communities
FET H2020FET OPEN: FET-Open research and innovation actions
FET H2020FET OPEN: Capturing non-Amplified Tumor Circulating DNA with Ultrasound Hydrodynamics
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publication . Other literature type . Article . 2019

3D-printed Point-of-Care Platform for Genetic Testing of Infectious Diseases Directly in Human Samples Using Acoustic Sensors and a Smartphone

George Papadakis; Alexandros K. Pantazis; Maria Ntogka; Konstantinos Parasyris; Gesthimani-Ioanna Theodosi; Georgia Kaprou; Electra Gizeli;