publication . Other literature type . Article . 2016

Variation in Fumonisin and Ochratoxin Production Associated with Differences in Biosynthetic Gene Content in Aspergillus niger and A. welwitschiae Isolates from Multiple Crop and Geographic Origins

Susca, Antonia; Proctor, Robert H.; Morelli, Massimiliano; Haidukowski, Miriam; Gallo, Antonia; Logrieco, Antonio F.; Moretti, Antonio;
  • Published: 09 Sep 2016
  • Publisher: Frontiers Media SA
Abstract
The fungi Aspergillus niger and A. welwitschiae are morphologically indistinguishable species used for industrial fermentation and for food and beverage production. The fungi also occur widely on food crops. Concerns about their safety have arisen with the discovery that some isolates of both species produce fumonisin (FB) and ochratoxin A (OTA) mycotoxins. Here, we examined FB and OTA production as well as the presence of genes responsible for synthesis of the mycotoxins in a collection of 92 A. niger/A. welwitschiae isolates from multiple crop and geographic origins. The results indicate that (i) isolates of both species differed in ability to produce the myco...
Subjects
Medical Subject Headings: food and beveragesfungi
free text keywords: fum cluster, ota cluster,fumonisin,ochratoxin,biosyntheticgenecluster, Aspergillusniger, Aspergillus welwitschiae, Microbiology, Original Research, fum cluster, ota cluster, fumonisin, ochratoxin, biosynthetic gene cluster, Aspergillus niger, Aspergillus welwitschiae, QR1-502, chemistry.chemical_compound, chemistry, Relative species abundance, Phylogenetic tree, Biology, Gene, Mycotoxin, Ochratoxin A, biology.organism_classification
Funded by
EC| MycoKey
Project
MycoKey
Integrated and innovative key actions for mycotoxin management in the food and feed chain
  • Funder: European Commission (EC)
  • Project Code: 678781
  • Funding stream: H2020 | RIA
40 references, page 1 of 3

Abbas H. K. Zablotowicz R. M. Bruns H. A. Abel C. A. (2006). Biocontrol of aflatoxin in corn by inoculation with non-aflatoxigenic Aspergillus flavus isolates. Biocontrol. Sci. Technol. 16, 437–449. 10.1080/09583150500532477 [OpenAIRE] [DOI]

Alexander N. J. Proctor R. H. McCormick S. P. (2009). Genes, gene clusters, and biosynthesis of trichothecenes and fumonisins in Fusarium. Toxin Rev. 28, 198–215. 10.1080/15569540903092142 [OpenAIRE] [DOI]

Altschul S. F. Madden T. L. Schaffer A. A. Zhang J. Zhang Z. Millwer W. . (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402. 9254694 [OpenAIRE] [PubMed]

Andersen M. R. Salazar M. P. Schaap P. J. van de Vondervoort P. J. I. Culley D. Thykaer J . (2011). Comparative genomics of citric-acid-producing Aspergillu s niger ATCC 1015 versus enzyme-producing CBS 513.88. Genome Res. 21, 885–897. 10.1101/gr.112169.110 21543515 [OpenAIRE] [PubMed] [DOI]

Baker S. E. (2006). Aspergillus niger genomics: past, present and into the future. Med. Mycol. 44, S17–S21. 10.1080/13693780600921037 [OpenAIRE] [DOI]

Battilani P. Barbano C. Marin S. Sanchis V. Kozakiewicz Z. Magan N. (2006). Mapping of Aspergillus section Nigri in Southern Europe and Israel based on geostatistical analysis. Int. J. Food Microbiol. 111, S72–S82. 10.1016/j.ijfoodmicro.2006.03.014 16737756 [OpenAIRE] [PubMed] [DOI]

Brakhage A. A. (2013). Regulation of fungal secondary metabolism. Nat. Rev. Microbiol. 11, 21–32. 10.1038/nrmicro2916 23178386 [OpenAIRE] [PubMed] [DOI]

Brown D. W. Butchko R. A. E. Busman M. Proctor R. H. (2007). The Fusarium verticillioides FUM gene cluster encodes a Zn(II)2Cys6 protein that affects FUM gene expression and fumonisin production. Eukaryot. Cell 6, 1210–1218. 10.1128/EC.00400-06 17483290 [OpenAIRE] [PubMed] [DOI]

Dorner J. W. Horn B. W. (2007). Separate and combined applications of nontoxigenic Aspergillus flavus and A. parasiticus for biocontrol of aflatoxin in peanuts. Mycopathologia 163, 215–223. 10.1007/s11046-007-9004-0 17390234 [OpenAIRE] [PubMed] [DOI]

Ferracin L. M. Fier C. B. Vieira M. L. C. Monteiro-Vitorello C. B. Varani Ade M. Rossi M. M. . (2012). Strain-specific polyketide synthase genes of Aspergillus niger. Int. J. Food Microbiol. 155, 137–145. 10.1016/j.ijfoodmicro.2012.01.020 22341916 [OpenAIRE] [PubMed] [DOI]

Frisvad J. C. Larsen T. O. Thrane U. Meijer M. Varga J. Samson R. A. . (2011). Fumonisin and ochratoxin production in industrial Aspergillus niger strains. PLoS ONE 6:e23496. 10.1371/journal.pone.0023496 21853139 [OpenAIRE] [PubMed] [DOI]

Frisvad J. C. Smedsgaard J. Samson R. A. Larsen T. O. Thrane U. (2007). Fumonisin B 2 production by Aspergillus niger. J. Agric. Food Chem. 55, 9727–9732. 10.1021/jf0718906 17929891 [OpenAIRE] [PubMed] [DOI]

Gallo A. Bruno K. S. Solfrizzo M. Perrone G. Mulè G. Visconti A. . (2012). New insight into the ochratoxin A biosynthetic pathway through deletion of a nonribosomal peptide synthetase gene in Aspergillus carbonarius. Appl. Environ. Microbiol. 78, 8208–8218. 10.1128/AEM.02508-12 22983973 [OpenAIRE] [PubMed] [DOI]

Gallo A. Knox B. P. Bruno K. S. Solfrizzo M. Baker S. E. Perrone G. (2014). Identification and characterization of the polyketide synthase involved in ochratoxin A biosynthesis in Aspergillus carbonarius. Int. J. Food Microbiol. 179, 10–17. 10.1016/j.ijfoodmicro.2014.03.013 24699234 [OpenAIRE] [PubMed] [DOI]

Gallo A. Perrone G. Solfrizzo M. Epifani F. Abbas A. Dobson A. D. . (2009). Characterisation of a pks gene which is expressed during ochratoxin A production by Aspergillus carbonarius. Int. J. Food Microbiol. 129, 8–15. 10.1016/j.ijfoodmicro.2008.10.022 19038469 [OpenAIRE] [PubMed] [DOI]

40 references, page 1 of 3
Abstract
The fungi Aspergillus niger and A. welwitschiae are morphologically indistinguishable species used for industrial fermentation and for food and beverage production. The fungi also occur widely on food crops. Concerns about their safety have arisen with the discovery that some isolates of both species produce fumonisin (FB) and ochratoxin A (OTA) mycotoxins. Here, we examined FB and OTA production as well as the presence of genes responsible for synthesis of the mycotoxins in a collection of 92 A. niger/A. welwitschiae isolates from multiple crop and geographic origins. The results indicate that (i) isolates of both species differed in ability to produce the myco...
Subjects
Medical Subject Headings: food and beveragesfungi
free text keywords: fum cluster, ota cluster,fumonisin,ochratoxin,biosyntheticgenecluster, Aspergillusniger, Aspergillus welwitschiae, Microbiology, Original Research, fum cluster, ota cluster, fumonisin, ochratoxin, biosynthetic gene cluster, Aspergillus niger, Aspergillus welwitschiae, QR1-502, chemistry.chemical_compound, chemistry, Relative species abundance, Phylogenetic tree, Biology, Gene, Mycotoxin, Ochratoxin A, biology.organism_classification
Funded by
EC| MycoKey
Project
MycoKey
Integrated and innovative key actions for mycotoxin management in the food and feed chain
  • Funder: European Commission (EC)
  • Project Code: 678781
  • Funding stream: H2020 | RIA
40 references, page 1 of 3

Abbas H. K. Zablotowicz R. M. Bruns H. A. Abel C. A. (2006). Biocontrol of aflatoxin in corn by inoculation with non-aflatoxigenic Aspergillus flavus isolates. Biocontrol. Sci. Technol. 16, 437–449. 10.1080/09583150500532477 [OpenAIRE] [DOI]

Alexander N. J. Proctor R. H. McCormick S. P. (2009). Genes, gene clusters, and biosynthesis of trichothecenes and fumonisins in Fusarium. Toxin Rev. 28, 198–215. 10.1080/15569540903092142 [OpenAIRE] [DOI]

Altschul S. F. Madden T. L. Schaffer A. A. Zhang J. Zhang Z. Millwer W. . (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402. 9254694 [OpenAIRE] [PubMed]

Andersen M. R. Salazar M. P. Schaap P. J. van de Vondervoort P. J. I. Culley D. Thykaer J . (2011). Comparative genomics of citric-acid-producing Aspergillu s niger ATCC 1015 versus enzyme-producing CBS 513.88. Genome Res. 21, 885–897. 10.1101/gr.112169.110 21543515 [OpenAIRE] [PubMed] [DOI]

Baker S. E. (2006). Aspergillus niger genomics: past, present and into the future. Med. Mycol. 44, S17–S21. 10.1080/13693780600921037 [OpenAIRE] [DOI]

Battilani P. Barbano C. Marin S. Sanchis V. Kozakiewicz Z. Magan N. (2006). Mapping of Aspergillus section Nigri in Southern Europe and Israel based on geostatistical analysis. Int. J. Food Microbiol. 111, S72–S82. 10.1016/j.ijfoodmicro.2006.03.014 16737756 [OpenAIRE] [PubMed] [DOI]

Brakhage A. A. (2013). Regulation of fungal secondary metabolism. Nat. Rev. Microbiol. 11, 21–32. 10.1038/nrmicro2916 23178386 [OpenAIRE] [PubMed] [DOI]

Brown D. W. Butchko R. A. E. Busman M. Proctor R. H. (2007). The Fusarium verticillioides FUM gene cluster encodes a Zn(II)2Cys6 protein that affects FUM gene expression and fumonisin production. Eukaryot. Cell 6, 1210–1218. 10.1128/EC.00400-06 17483290 [OpenAIRE] [PubMed] [DOI]

Dorner J. W. Horn B. W. (2007). Separate and combined applications of nontoxigenic Aspergillus flavus and A. parasiticus for biocontrol of aflatoxin in peanuts. Mycopathologia 163, 215–223. 10.1007/s11046-007-9004-0 17390234 [OpenAIRE] [PubMed] [DOI]

Ferracin L. M. Fier C. B. Vieira M. L. C. Monteiro-Vitorello C. B. Varani Ade M. Rossi M. M. . (2012). Strain-specific polyketide synthase genes of Aspergillus niger. Int. J. Food Microbiol. 155, 137–145. 10.1016/j.ijfoodmicro.2012.01.020 22341916 [OpenAIRE] [PubMed] [DOI]

Frisvad J. C. Larsen T. O. Thrane U. Meijer M. Varga J. Samson R. A. . (2011). Fumonisin and ochratoxin production in industrial Aspergillus niger strains. PLoS ONE 6:e23496. 10.1371/journal.pone.0023496 21853139 [OpenAIRE] [PubMed] [DOI]

Frisvad J. C. Smedsgaard J. Samson R. A. Larsen T. O. Thrane U. (2007). Fumonisin B 2 production by Aspergillus niger. J. Agric. Food Chem. 55, 9727–9732. 10.1021/jf0718906 17929891 [OpenAIRE] [PubMed] [DOI]

Gallo A. Bruno K. S. Solfrizzo M. Perrone G. Mulè G. Visconti A. . (2012). New insight into the ochratoxin A biosynthetic pathway through deletion of a nonribosomal peptide synthetase gene in Aspergillus carbonarius. Appl. Environ. Microbiol. 78, 8208–8218. 10.1128/AEM.02508-12 22983973 [OpenAIRE] [PubMed] [DOI]

Gallo A. Knox B. P. Bruno K. S. Solfrizzo M. Baker S. E. Perrone G. (2014). Identification and characterization of the polyketide synthase involved in ochratoxin A biosynthesis in Aspergillus carbonarius. Int. J. Food Microbiol. 179, 10–17. 10.1016/j.ijfoodmicro.2014.03.013 24699234 [OpenAIRE] [PubMed] [DOI]

Gallo A. Perrone G. Solfrizzo M. Epifani F. Abbas A. Dobson A. D. . (2009). Characterisation of a pks gene which is expressed during ochratoxin A production by Aspergillus carbonarius. Int. J. Food Microbiol. 129, 8–15. 10.1016/j.ijfoodmicro.2008.10.022 19038469 [OpenAIRE] [PubMed] [DOI]

40 references, page 1 of 3
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