publication . Article . 2012

Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium.

Marcel Felder; Beat Haenni; Thomas Geiser; Olivier Thierry Guenat; Laurent Barbe; Amiq Gazdhar; Pauline Sallin;
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  • Published: 01 Jan 2012 Journal: Lab Chip, volume 12, page 640
Abstract
We present a microfluidic epithelial wound-healing assay that allows characterization of the effect of hepatocyte growth factor (HGF) on the regeneration of alveolar epithelium using a flow-focusing technique to create a regular wound in the epithelial monolayer. The phenotype of the epithelial cell was characterized using immunostaining for tight junction (TJ) proteins and transmission electron micrographs (TEMs) of cells cultured in the microfluidic system, a technique that is reported here for the first time. We demonstrate that alveolar epithelial cells cultured in a microfluidic environment preserve their phenotype before and after wounding. In addition, we...
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doi: 10.1039/c1lc20879a
Subjects
free text keywords: Biochemistry, Bioengineering, General Chemistry, Biomedical Engineering, Microfluidics, Phenotype, Cell biology, Tight junction, Alveolar Epithelium, Epithelium, medicine.anatomical_structure, medicine, Biology, Hepatocyte growth factor, medicine.drug, Cell culture, Immunostaining
Funded by
SNSF| Alveolar-capillary microfluidic model for the analysis of lung injury, regeneration and repair
Project
Alveolar-capillary microfluidic model for the analysis of lung injury, regeneration and repair
  • Funder: Swiss National Science Foundation (SNSF)
  • Project Code: 315230_141127
  • Funding stream: Project funding | Project funding (Div. I-III)
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