publication . Article . 1995

Electron Leakage from the Adrenal-Cortex Mitochondrial P450Scc and P450C11 Systems: NADPH and Steroid Dependence

David Sklan; Israel Hanukoglu; R. Rapoport;
Open Access
  • Published: 01 Mar 1995
  • Publisher: Elsevier BV
Abstract
In the present study we examined the coupling of NADPH oxidation to substrate hydroxylation and the effects of steroids on this process in reconstituted P450scc and P450c11 systems. To determine the relative rates of substrate hydroxylation vs electron leakage we assayed both the steroid product and H2O2 in the same sample. For both P450 systems the rates of steroid product and superoxide formation increased as NADPH concentration was increased. However, P450c11 was found to be more leaky. The leakage from the P450scc system was not affected by pregnenolone, the product of cholesterol side chain cleavage. In contrast, corticosterone, the product of P450c11, incr...
Subjects
free text keywords: Biophysics, Biochemistry, Molecular Biology, Endocrinology, medicine.medical_specialty, medicine, Chemistry, Cholesterol side-chain cleavage enzyme, Steroid, medicine.medical_treatment, Corticosterone, chemistry.chemical_compound, Hydroxylation, Superoxide, Pregnenolone, medicine.drug, Steroid 11-beta-hydroxylase, Internal medicine, NADPH oxidation
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