publication . Other literature type . Article . 2011

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster, Pinctada margaritifera

Gilles Le Moullac;
Open Access English
  • Published: 01 Apr 2011
  • Publisher: Edp Sciences S A
  • Country: France
Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the de- velopment of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry. Spermatozoa were cryopreserved with cryoprotectant agent (CPA) 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and st...
Medical Subject Headings: urogenital systemendocrine systemreproductive and urinary physiology
free text keywords: Cryopreservation, Spermatozoa, Fertility, Larval rearing, Pearl oyster, Pinctada margaritifera, Aquatic Science
29 references, page 1 of 2

Acosta-Salmón H., Jerry D.J., Southgate P.C., 2007, Effects of cryoprotectant agents and freezing protocol on motility of black-lip pearl oyster (Pinctada margaritifera L.) spermatozoa. Cryobiology 54, 13-18.

Adams S.L., Hessian P.A., Mladenov P.V., 2003, Flow cytometric evaluation of mitochondrial function and membrane integrity of marine invertebrate sperm. Invertebr. Reprod. Dev. 44, 45-51. [OpenAIRE]

Adams S.L., Hessian P.A., Mladenov P.V., 2004a, Cryopreservation of sea urchin (Evechinus chloroticus) sperm. Cryo Lett. 25, 287- 300.

Adams S.L., Smith J.F., Roberts R.D., Janke A.R., Kaspar H.F., Tervit H.R., Pugh P.A., Webb S.C., King N.G., 2004b, Cryopreservation of sperm of the Pacific Oyster (Crassostrea gigas): development of a practical method for commercial spat production. Aquaculture 242, 271-282.

Adams S.L., Smith J.F., Roberts R.D., Janke A.R., King N.G., Tervit H.R., Webb S.C., 2008, Application of sperm cryopreservation in selective breeding of the Pacific oyster, Crassostrea gigas (Thunberg). Aquac. Res. 39, 1434-1442.

Arnaud-Haond S., Goyard E., Vonau V., Herbaut C., Prou J., Saulnier D., 2007, Pearl Formation : Persistence of the Graft During the Entire Process of biomineralization. Mar. Biotechnol. 9, 113- 116. [OpenAIRE]

Bougrier S., Rabenomanana L.D., 1986, Cryopreservation of spermatozoa of the Japanese Oyster, Crassostrea gigas. Aquaculture 58, 277-280.

Cabrita E., Robles V., Cuñado S., Wallace J.C., Sarasquete C., Herráez, M.P., 2005, Evaluation of gilthead sea bream, Sparus aurata, sperm quality after cryopreservation in 5ml macrotubes. Cryobiology 50, 273-284. [OpenAIRE]

Fauvel C., Suquet M., Dreanno C., Zonno V., Menu B., 1998, Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production stimulating conditions. Aquat. Living Resour. 11, 387-394. [OpenAIRE]

Flajshans M., Cosson J., Rodina M., Linhart O., 2004, The application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa. Cell Biol. Inter. 28, 955- 959.

Graham J.K., Kunze E., Hammerstedt R.H., 1990, Analysis of sperm cell viability, acrosomal integrity, and mitochondrial function using flow cytometry. Biol. Reprod. 43, 55-64. [OpenAIRE]

Grzyb K., Rychlowski M., Biegniewska A.,. Skorkowski E.F., 2003, Quantitative determination of creatine kinase release from herring (Clupea harengus) spermatozoa induced by tributyltin. Comp. Biochem. Physiol. C 134, 207-213. [OpenAIRE]

Gwo J.C., Wu C.Y., Chang W.S.P., Cheng H.Y., 2003, Evaluation of damage in Pacific oyster (Crassostrea gigas) spermatozoa before and after cryopreservation using comet assay. Cryo. Lett. 24, 171-180.

Heasman M.P., O'Connor W.A., Frazer A.W., 1996, Effects of fertilization and incubation factors on the quality and yield of scallop, Pecten fumatus Reeve larvae. Aquac. Res. 27, 505-513.

Horvat A., Wayman W.R., Dean J.C., Urbanyi B., Tiersch T.R., Mims S.D., Johnson D., Jenkins J.A., 2008, Viability and fertilizing capacity of cryopreserved sperm from three North American acipenseriform species: a retrospective study. J. Appl. Ichthyol. 24, 443-449. [OpenAIRE]

29 references, page 1 of 2
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