publication . Other literature type . Article . 2011

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster, Pinctada margaritifera

Gilles Le Moullac;
Open Access English
  • Published: 01 Apr 2011
  • Publisher: Edp Sciences S A
  • Country: France
Abstract
Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the de- velopment of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry. Spermatozoa were cryopreserved with cryoprotectant agent (CPA) 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and st...
Subjects
Medical Subject Headings: urogenital systemendocrine systemreproductive and urinary physiology
free text keywords: Cryopreservation, Spermatozoa, Fertility, Larval rearing, Pearl oyster, Pinctada margaritifera, Aquatic Science
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