RNA-guided editing of bacterial genomes using CRISPR-Cas systems

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Jiang, Wenyan; Bikard, David; Cox, David Daniel; Zhang, Feng; Marraffini, Luciano A.;

Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations in the genomes of Streptococcus pneumoniae and Escherichia coli. The approach relies on dual-RNA:Cas... View more
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