publication . Preprint . 2018

Moving microfluidics ahead: Extending capabilities, accessibility, and applications

Blainey, Paul;
Open Access English
  • Published: 10 Jan 2018
Paul Blainey is professor of Biological Engineering at MIT. In this contribution he describes three microfluidic technologies that he and his team has developed to extend the capability, accessibility, and applications of microfluidics: (1) Integrated microfluidic sample preparation for genomic assays, (2) hydrogel-based microfluidics for single-cell genome sequencing, and (3) an emulsion-based system for combinatorial drug screening.
free text keywords: Physics - Fluid Dynamics, Quantitative Biology - Other Quantitative Biology
Download from

T.   Thorsen,   S.   J.   Maerkl,   S.   R.   Quake,   Microfluidic   large-­‐scale   integration.   Science   298,  580  (2002).  

J.   Bibette,   D.   Morse,   T.   Witten,   D.   Weitz,   Stability   criteria   for   emulsions.   Phys  Rev   Lett  69,  2439  (1992).  

S.   Kim   et   al.,   High-­‐throughput   automated   microfluidic   sample   preparation   for   accurate  microbial  genomics.  Nature  Communications  8,  13919  (2017).   J.   Melin,   S.   R.   Quake,   Microfluidic   large-­‐scale   integration:   the   evolution   of   design   rules  for  biological  automation.  Annu.  Rev.  Biophys.  Biomol.  Struct.  36,  213  (2007).   H.-­‐P.   Chou,   M.   A.   Unger,   S.   R.   Quake,   A   microfabricated   rotary   pump.   Biomedical   Microdevices  3,  323  (2001).  

R.  D.  Mitra,  G.  M.  Church,  In  situ  localized  amplification  and  contact  replication  of   many  individual  DNA  molecules.  Nucleic  Acids  Res  27,  e34  (Dec  15,  1999).   L.   Xu,   I.   L.   Brito,   E.   J.   Alm,   P.   C.   Blainey,   Virtual   microfluidics   for   digital   quantification  and  single-­‐cell  sequencing.  Nature  Methods,    (2016).   R.   Lasken,   T.   Stockwell,   Mechanism   of   chimera   formation   during   the   Multiple   Displacement  Amplification  reaction.  BMC  biotechnology  7,  19  (2007).   P.  Imming,  C.  Sinning,  A.  Meyer,  Drugs,  their  targets  and  the  nature  and  number  of   drug  targets.  Nature  reviews  Drug  discovery  5,  821  (2006).   Y.   Chen,   A.   W.   Gani,   S.   K.   Tang,   Characterization   of   sensitivity   and   specificity   in   leaky  droplet-­‐based  assays.  Lab  on  a  Chip  12,  5093  (2012).   M.   Pan,   F.   Lyu,   S.   K.   Tang,   Fluorinated   Pickering   Emulsions   with   Nonadsorbing   Interfaces   for   Droplet-­‐based   Enzymatic   Assays.   Analytical   chemistry   87,   7938   (2015).  

P.  Umbanhowar,  V.  Prasad,  D.  Weitz,  Monodisperse  emulsion  generation  via  drop   break  off  in  a  coflowing  stream.  Langmuir  16,  347  (2000).   [OpenAIRE]

Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue