publication . Article . 2015

Phosphorylation and Dephosphorylation of the Presequence of Precursor MULTIPLE ORGANELLAR RNA EDITING FACTOR3 during Import into Mitochondria from Arabidopsis

Name: Phosphorylation and Dephosphorylation of the Presequence of Precursor MULTIPLE ORGANELLAR RNA EDITING FACTOR3 during Import into Mitochondria from Arabidopsis
Keywords: Plant Science, Genetics, Physiology

Shifeng Cheng; Boon Leong Lim; James Whelan; Monika W. Murcha; Yee-Song Law; Renshan Zhang; Feng Sun; Xiaoqian Guan; Owen Duncan;

Open Access English

Published: 24 Aug 2015

Country: China (People's Republic of)

Summary

Abstract

The nucleus-encoded mitochondria-targeted proteins, multiple organellar RNA editing factors (MORF3, MORF5, and MORF6), interact with Arabidopsis (Arabidopsis thaliana) PURPLE ACID PHOSPHATASE2 (AtPAP2) located on the chloroplast and mitochondrial outer membranes in a presequence-dependent manner. Phosphorylation of the presequence of the precursor MORF3 (pMORF3) by endogenous kinases in wheat germ translation lysate, leaf extracts, or STY kinases, but not in rabbit reticulocyte translation lysate, resulted in the inhibition of protein import into mitochondria. This inhibition of import could be overcome by altering threonine/serine residues to alanine on the pre...

doi: 10.1104/pp.15.01115

Subjects

Medical Subject Headings: food and beverages

free text keywords: Plant Science, Genetics, Physiology, Biochemistry, Transport protein, RNA editing, Kinase activity, Dephosphorylation, Biology, Phosphorylation, Kinase, Chloroplast, Protein-Serine-Threonine Kinases

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