publication . Article . Other literature type . 2012

Comparison of buffers for extraction of mite allergen der p 1 from dust

Prester, Ljerka; Kovačić, Jelena; Macan, Jelena;
Open Access
  • Published: 25 Sep 2012 Journal: Archives of Industrial Hygiene and Toxicology, volume 63, pages 293-300 (issn: 0004-1254, Copyright policy)
  • Publisher: Walter de Gruyter GmbH
Abstract
Der p 1 is the main allergen of house dust mite Dermatophagoides pteronyssinus, which has routinely been detected in residential dust. However, the procedure for extracting Der p 1 from reservoir dust has not been well defined. The aim of this study was to compare Der p 1 mass fractions in dust extracts prepared using the following extraction buffers: phosphate (pH 7.4), borate (pH 8.0), and ammonium bicarbonate (pH 8.0), all with 0.05 % Tween 20. Twenty-eight dust samples were divided into three aliquots and each portion was extracted with one of the three buffers at room temperature. Der p 1 mass fractions were measured in a total of 84 dust extracts using the...
Subjects
Medical Subject Headings: respiratory tract diseases
free text keywords: Toxicology, Public Health, Environmental and Occupational Health, Mite allergen, Bovine serum albumin, biology.protein, biology, Chromatography, Bicarbonate, chemistry.chemical_compound, chemistry, Phosphate, Medicine, business.industry, business, Dermatophagoides pteronyssinus; ELISA; extraction buffers; indoor allergens; intraclass correlation; settled dust, alergeni unutarnjih prostora; Dermatophagoides pteronyssinus; ekstrakcijski puferi; ELISA; “intraclass” korelacija, sedimentirana prašina
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publication . Article . Other literature type . 2012

Comparison of buffers for extraction of mite allergen der p 1 from dust

Prester, Ljerka; Kovačić, Jelena; Macan, Jelena;