publication . Other literature type . Article . 2017 . Embargo end date: 01 Jan 2017

The effect of wound dressings on a bio-engineered human dermo-epidermal skin substitute in a rat model

Martina Hüging; Thomas Biedermann; Monia Sobrio; Sarah A. Meyer; Sophie Böttcher-Haberzeth; Edith Manuel; Maya Horst; Sally Hynes; Ernst Reichmann; Clemens Schiestl; ...
Open Access
  • Published: 01 Jan 2017
  • Publisher: Lippincott Williams & Wilkins
  • Country: Switzerland
Abstract
Autologous bio-engineered dermo-epidermal skin substitutes are a promising treatment for large skin defects such as burns. For their successful clinical application, the graft dressing must protect and support the keratinocyte layer and, in many cases, possess antimicrobial properties. However, silver in many antimicrobial dressings may inhibit keratinocyte growth and differentiation. The purpose of our study was to evaluate the effect of various wound dressings on the healing of a human hydrogel-based dermo-epidermal skin substitute in preparation for the first-in-human clinical trials. Human dermo-epidermal skin substitutes approved for clinical trials were pr...
Subjects
Medical Subject Headings: integumentary system
free text keywords: Clinic for Surgery, 610 Medicine & health, Random allocation, Rat model, Graft survival, Vaseline, Clinical trial, Medicine, business.industry, business, Wound healing, Skin substitutes, Keratinocyte, medicine.anatomical_structure, Dermatology, medicine.medical_specialty
Related Organizations
Funded by
EC| MULTITERM
Project
MULTITERM
Training Multidisciplinary scientists for Tissue Engineering and Regenerative Medicine
  • Funder: European Commission (EC)
  • Project Code: 238551
  • Funding stream: FP7 | SP3 | PEOPLE
,
EC| EUROSKINGRAFT
Project
EUROSKINGRAFT
A novel generation of skin substitutes to clinically treat a broad spectrum of severe skin defects
  • Funder: European Commission (EC)
  • Project Code: 279024
  • Funding stream: FP7 | SP1 | HEALTH
58 references, page 1 of 4

1. Rheinwald JG, Green H. Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature 1977;265:421-4. [OpenAIRE]

2. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 1975;6:331-43.

3. Gallico GG 3rd, O'Connor NE, Compton CC, Kehinde O, Green H. Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med 1984;311:448-51. [OpenAIRE]

4. Boyce ST, Goretsky MJ, Greenhalgh DG, Kagan RJ, Rieman MT, Warden GD. Comparative assessment of cultured skin substitutes and native skin autograft for treatment of fullthickness burns. Ann Surg 1995;222:743-52. [OpenAIRE]

5. Boyce ST, Kagan RJ, Greenhalgh DG, et al. Cultured skin substitutes reduce requirements for harvesting of skin autograft for closure of excised, full-thickness burns. J Trauma 2006;60:821-9.

6. Boyce ST, Simpson PS, Rieman MT, et al. Randomized, paired-site comparison of autologous engineered skin substitutes and split-thickness skin graft for closure of extensive, full-thickness burns. J Burn Care Res 2017. doi:10.1097/ BCR.0000000000000401.

7. Shakespeare PG. The role of skin substitutes in the treatment of burn injuries. Clin Dermatol 2017;23:413-8.

8. Edmonds M. Apligraf in the treatment of neuropathic diabetic foot ulcers. Int J Low Extrem Wounds 2009;8:11-8.

9. Barber C, Watt A, Pham C, et al. Influence of bioengineered skin substitutes on diabetic foot ulcer and venous leg ulcer outcomes. J Wound Care 2008;17:517-27.

10. Waymack P, Duff RG, Sabolinski M. The effect of a tissue engineered bilayered living skin analog, over meshed splitthickness autografts on the healing of excised burn wounds. The Apligraf Burn Study Group. Burns 2000;26:609-19.

11. Pontiggia L, Biedermann T, Meuli M, et al. Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation. J Invest Dermatol 2009;129:480-90.

12. Braziulis E, Diezi M, Biedermann T, et al. Modified plastic compression of collagen hydrogels provides an ideal matrix for clinically applicable skin substitutes. Tissue Eng Part C Methods 2012;18:464-74. [OpenAIRE]

13. Hartmann-Fritsch F, Biedermann T, Braziulis E, Meuli M, Reichmann E. A new model for preclinical testing of dermal substitutes for human skin reconstruction. Pediatr Surg Int 2013;29:479-88.

14. Biedermann T, Klar AS, Böttcher-Haberzeth S, Schiestl C, Reichmann E, Meuli M. Tissue-engineered dermo-epidermal skin analogs exhibit de novo formation of a near natural neurovascular link 10 weeks after transplantation. Pediatr Surg Int 2014;30:165-72.

15. Böttcher-Haberzeth S, Biedermann T, Klar AS, et al. Characterization of pigmented dermo-epidermal skin substitutes in a long-term in vivo assay. Exp Dermatol 2015;24:16-21.

58 references, page 1 of 4
Any information missing or wrong?Report an Issue