publication . Article . Other literature type . 2011

Extracellular Hemoglobin Polarizes the Macrophage Proteome toward Hb-Clearance, Enhanced Antioxidant Capacity and Suppressed HLA Class 2 Expression

Kaempfer Theresa; Duerst Elena; Gehrig Peter; Roschitzki Bernd; Rutishauser Dorothea; Grossmann Jonas; Schoedon Gabriele; Vallelian Florence; Schaer Dominik J;
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  • Published: 06 May 2011 Journal: Journal of Proteome Research, volume 10, pages 2,397-2,408 (issn: 1535-3893, eissn: 1535-3907, Copyright policy)
  • Publisher: American Chemical Society (ACS)
  • Country: Switzerland
Abstract
Peripheral blood monocytes and macrophages are the only cell population with a proven hemoglobin (Hb) clearance capacity through the CD163 scavenger receptor pathway. Hb detoxification and related adaptive cellular responses are assumed to be essential processes to maintaining tissue homeostasis and promoting wound healing in injured tissues. Using a dual platform mass spectrometry analysis with MALDI-TOF/TOF and LTQ-Orbitrap instruments combined with isobaric tag for relative and absolute quantitation (iTRAQ), we analyzed how Hb exposure could modulate the macrophage phenotype on a proteome level. We identified and relatively quantified 3691 macrophage proteins...
Subjects
free text keywords: Institute of Anatomy, Clinic and Policlinic for Internal Medicine, Functional Genomics Center Zurich, 570 Life sciences; biology, 610 Medicine & health, Biochemistry, General Chemistry, Molecular biology, Biology, Hemoglobin, Scavenger receptor, Isobaric tag for relative and absolute quantitation, Monocyte, medicine.anatomical_structure, medicine, Population, education.field_of_study, education, Extracellular, CD163, Proteome
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