publication . Other literature type . Conference object . 2011 . Embargo end date: 02 Sep 2011

Fluorescence Image Segmentation by using Digitally Reconstructed Fluorescence Images

Blumer, Clemens; Vivien, Cyprien; Oertner, Thomas G; Vetter, Thomas;
Open Access
  • Published: 02 Sep 2011
  • Publisher: s.n.
  • Country: Switzerland
Abstract
In biological experiments fluorescence imaging is used to image living and stimulated neurons. But the analysis of fluorescence images is a difficult task. It is not possible to conclude the shape of an object from fluorescence images alone. Therefore, it is not feasible to get good manual segmented nor ground truth data from fluorescence images. Supervised learning approaches are not possible without training data. To overcome this issues we propose to synthesize fluorescence images and call them 'Digitally Reconstructed Fluorescence Images'(DRFI). We propose how DRFIs are computed with data from ’Serial Block-Face Scanning Electron Microscopy’ (SBFS-EM). As no...
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free text keywords: SystemsX.ch, Interdisciplinary PhD Projects, 570 Life sciences; biology
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