publication . Other literature type . Article . 1999

General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1α

Gieri Camenisch; Mauro Tini; Dmitri Chilov; Ivica Kvietikova; Vickram Srinivas; Jaime Caro; Patrick Spielmann; Roland H. Wenger; Max Gassmann;
Open Access
  • Published: 01 Jan 1999
  • Publisher: Wiley
  • Country: Switzerland
Abstract
Avian embryos and neonates acquire passive immunity by transferring maternal immunoglobulins from serum to egg yolk. Despite being a convenient source of antibodies, egg yolk immunoglobulins (IgY) from immunized hens have so far received scant attention in research. Here we report the generation and rapid isolation of IgY from the egg yolk of hens immunized against the alpha subunit of the human hypoxia-inducible factor 1 (HIF-1alpha). Anti-HIF-1alpha IgY antibodies were affinity purified and tested for their performance in various applications. Abundant HIF-1alpha protein was detected by Western blot analysis in nuclear extracts derived from hypoxic cells of hu...
Subjects
free text keywords: Biotechnology, Genetics, Biochemistry, Molecular Biology, Institute of Physiology, 570 Life sciences; biology, Passive immunity, medicine.medical_treatment, medicine, Immunofluorescence, medicine.diagnostic_test, Antibody, biology.protein, biology, Chemistry, Affinity chromatography, Western blot, Monoclonal antibody, medicine.drug_class, Yolk, food.ingredient, food, Immunology, Polyclonal antibodies
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