publication . Thesis . 2009

Amino acid analysis in biological fluids by GC-MS

Name: Amino acid analysis in biological fluids by GC-MS
Creator: Kaspar, Hannelore

Kaspar, Hannelore;

Open Access English

Published: 06 Aug 2009

Country: Germany

Summary
references
66

Abstract

Amino acids are intermediates in cellular metabolism and their quantitative analysis plays an important role in disease diagnostics. A gas chromatography-mass spectrometry (GC-MS) based method was developed for the quantitative analysis of free amino acids as their propyl chloroformate derivatives in biological fluids. Derivatization with propyl chloroformate could be carried out directly in the biological samples without prior protein precipitation or solid-phase extraction of the amino acids, thereby allowing for automation of the entire procedure, including addition of reagents, extraction and injection into the GC-MS. The total analysis time was 30 minutes, ...

Subjects

free text keywords: 540 Chemie, ddc:540

Related Organizations

University of Regensburg
Germany

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Thesis . 2009

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