Radial oxygen gradients over rat cortex arterioles
610 Medizin | ddc:610
We present the results of the visualisation of radial oxygen gradients in rats’ cortices and their use in neurocritical management.
PO2 maps of the cortex of 10 wistar rats were obtained with a camera (SensiMOD, PCO, Kehlheim, Germany). Those pictures were analyzed and edited by a custom-made software. We chose a vessel for examination. A matrix, designed to evaluate the cortical O2 partial pressure, was placed vertically to the artery and afterwards multiple regions of interest were measured (width 10 pixels, length 15 - 50 pixels). The results showed a map of the cerebral oxygenation, which allowed us to calculate radial oxygen gradients over arterioles. Three groups were defined according to the level of the arterial pO2: apO2 <80, apO2 80-120 and apO2 >120 and were analysed statistically.
The gradient 1° showed significantly different cortical pO2 values between the three different groups. The mean pO2 value were 2,62 mmHg/mm, 5,29 mmHg/mm, 5,82 mmHg/mm. The gradient 2° showed less significant values, 0,56 mmHg/mm, 0,90 mmHg/mm to 1,02 mmHg/mm. But the last gradient 3° showed again significant results, 3,18 mmHg/mm, 6,19 mmHg/mm and 6,84 mmHg/mm.
Using these gradients, it is possible to describe and compare the distribution of oxygen to certain part of the brain, bringing forward a semi-invasive cortical O2-monitoring. We showed that it is also possible to detect pO2 changes in the oxygen supply of the brain.
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