Radial oxygen gradients over rat cortex arterioles

Doctoral thesis English OPEN
Galler, Michael (2011)
  • Subject: 610 Medizin | ddc:610

Purpose: We present the results of the visualisation of radial oxygen gradients in rats’ cortices and their use in neurocritical management. Methods: PO2 maps of the cortex of 10 wistar rats were obtained with a camera (SensiMOD, PCO, Kehlheim, Germany). Those pictures were analyzed and edited by a custom-made software. We chose a vessel for examination. A matrix, designed to evaluate the cortical O2 partial pressure, was placed vertically to the artery and afterwards multiple regions of interest were measured (width 10 pixels, length 15 - 50 pixels). The results showed a map of the cerebral oxygenation, which allowed us to calculate radial oxygen gradients over arterioles. Three groups were defined according to the level of the arterial pO2: apO2 <80, apO2 80-120 and apO2 >120 and were analysed statistically. Results: The gradient 1° showed significantly different cortical pO2 values between the three different groups. The mean pO2 value were 2,62 mmHg/mm, 5,29 mmHg/mm, 5,82 mmHg/mm. The gradient 2° showed less significant values, 0,56 mmHg/mm, 0,90 mmHg/mm to 1,02 mmHg/mm. But the last gradient 3° showed again significant results, 3,18 mmHg/mm, 6,19 mmHg/mm and 6,84 mmHg/mm. Conclusion: Using these gradients, it is possible to describe and compare the distribution of oxygen to certain part of the brain, bringing forward a semi-invasive cortical O2-monitoring. We showed that it is also possible to detect pO2 changes in the oxygen supply of the brain.
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