publication . Thesis . 2015

Analyse der F-box Funktion von Rca1 in Drosophila melanogaster

Frank, Martina;
Open Access German
  • Published: 19 Jan 2015
  • Country: Germany
Abstract
Rca1 ist ein wichtiger Inhibitor für die Ubiqutin-E3-Ligase APC/C mit dem Aktivatorproteine Fzr (Fizzy-related, ortholog zu Cdh1), vor allem in der G2-Phase des Zellzyklus, und ermöglicht die Akkumulati-on von Cyclinen für die darauffolgende Mitose. Embryonen, die die rca1-Allele rca1[2] und rca1[C1474] tragen, arretieren deshalb im Zellzyklus 16 und können nicht in die Mitose 16 eintreten. Durch Überexpression von Rca1 und Rca1 mit deletierter F-box (Rca1ΔF-box) konnte dieser Phänotyp komplementiert werden. In dieser Arbeit wurde die genomische Region von rca1 kloniert, um die Analyse mit endogenen Mengen von Rca1 und Rca1 ohne F-box weiterzuführen. Die Express...
Subjects
free text keywords: 570 Biowissenschaften, Biologie, ddc:570
Related Organizations
57 references, page 1 of 4

al., 1999; Blanco et al., 2000; Listovsky et al., 2000; Castro et al., 2002; Littlepage und Ruderman, Matsushime, H., Quelle, D.E., Shurtleff, S.A., Shibuya, M., Sherr, C.J., and Kato, J.Y. (1994). D-type cyclin-dependent kinase activity in mammalian cells. Mol Cell Biol 14, 2066-2076.

Matyskiela, M.E., and Morgan, D.O. (2009). Analysis of activator-binding sites on the APC/C supports a cooperative substrate-binding mechanism. Mol Cell 34, 68-80. [OpenAIRE]

McGarry, T.J., and Kirschner, M.W. (1998). Geminin, an inhibitor of DNA replication, is degraded during mitosis. Cell 93, 1043-1053. [OpenAIRE]

Merrill, P.T., Sweeton, D. and Wieschaus, E. (1988) Requirements for autosomal gene activity during precellular stages of Drosophila melanogaster. Development, 104, 495-509. [OpenAIRE]

(2006). Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitor. Genes Dev 20, 2410-2420.

Moberg, K.H., Bell, D.W., Wahrer, D.C., Haber, D.A., and Hariharan, I.K. (2001). Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines. Nature 413, 311-316. [OpenAIRE]

Moberg, K.H., Mukherjee, A., Veraksa, A., Artavanis-Tsakonas, S., and Hariharan, I.K. (2004). The Drosophila F box protein archipelago regulates dMyc protein levels in vivo. Current biology 14, 965-974. [OpenAIRE]

Montagnoli, A., Fiore, F., Eytan, E., Carrano, A.C., Draetta, G.F., Hershko, A., and Pagano, M. (1999).

Ubiquitination of p27 is regulated by Cdk-dependent phosphorylation and trimeric complex formation. Genes Dev 13, 1181-1189.

Morgan, D.O. (1995). Principles of CDK regulation. Nature 374, 131-134.

Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G., and Erlich, H. (1986). Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol 51 Pt 1, 263- 273. [OpenAIRE]

Musacchio, A., and Salmon, E.D. (2007). The spindle-assembly checkpoint in space and time. Nat Rev Mol Cell Biol 8, 379-393. [OpenAIRE]

Nakayama, K., Nagahama, H., Minamishima, Y.A., Miyake, S., Ishida, N., Hatakeyama, S., Kitagawa, M., Iemura, S., Natsume, T., and Nakayama, K.I. (2004). Skp2-mediated degradation of p27 regulates progression into mitosis. Developmental cell 6, 661-672.

Nishitani, H., Lygerou, Z., Nishimoto, T., and Nurse, P. (2000). The Cdt1 protein is required to license DNA for replication in fission yeast. Nature 404, 625-628. [OpenAIRE]

Nishitani, H., Sugimoto, N., Roukos, V., Nakanishi, Y., Saijo, M., Obuse, C., Tsurimoto, T., Nakayama, K.I., Nakayama, K., Fujita, M., et al. (2006a). Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis. EMBO J 25, 1126-1136.

57 references, page 1 of 4
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