publication . Thesis . 2015

Analyse der F-box Funktion von Rca1 in Drosophila melanogaster

Frank, Martina;
Open Access German
  • Published: 19 Jan 2015
  • Country: Germany
Rca1 ist ein wichtiger Inhibitor für die Ubiqutin-E3-Ligase APC/C mit dem Aktivatorproteine Fzr (Fizzy-related, ortholog zu Cdh1), vor allem in der G2-Phase des Zellzyklus, und ermöglicht die Akkumulati-on von Cyclinen für die darauffolgende Mitose. Embryonen, die die rca1-Allele rca1[2] und rca1[C1474] tragen, arretieren deshalb im Zellzyklus 16 und können nicht in die Mitose 16 eintreten. Durch Überexpression von Rca1 und Rca1 mit deletierter F-box (Rca1ΔF-box) konnte dieser Phänotyp komplementiert werden. In dieser Arbeit wurde die genomische Region von rca1 kloniert, um die Analyse mit endogenen Mengen von Rca1 und Rca1 ohne F-box weiterzuführen. Die Express...
free text keywords: 570 Biowissenschaften, Biologie, ddc:570
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