publication . Article . 2017

Accumulation of long-chain bases in yeast promotes their conversion to a long-chain base vinyl ether

Martínez-Montañés, Fernando; Lone, Museer A.; Hsu, Fong-Fu; Schneiter3, Roger;
Open Access English
  • Published: 27 Jan 2017
  • Country: Switzerland
Abstract
Long-chain bases (LCBs) are the precursors to ceramide and sphingolipids in eukaryotic cells. They are formed by the action of serine palmitoyl-CoA transferase (SPT), a complex of integral membrane proteins located in the endoplasmic reticulum. SPT activity is negatively regulated by Orm proteins to prevent the toxic overaccumulation of LCBs. Here we show that overaccumulation of LCBs in yeast results in their conversion to a hitherto undescribed LCB derivative, an LCB vinyl ether. The LCB vinyl ether is predominantly formed from phytosphingosine (PHS) as revealed by conversion of odd chain length tracers C17-dihydrosphingosine and C17- PHS into the correspondin...
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