publication . Article . Other literature type . 2017

Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae

Kazimirez Tarasiuk; Marylène Kobisch; Joachim Frey; Dominique Dugourd; J Harel; Bruno Chevallier; Marcelo Gottschalk;
Open Access
  • Published: 02 Aug 2017 Journal: FEMS Microbiology Letters, volume 160, pages 209-216 (issn: 0378-1097, eissn: 1574-6968, Copyright policy)
  • Publisher: Oxford University Press (OUP)
  • Country: Switzerland
Abstract
The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404±40 kb. The chromosome sizes for the reference strains of the other serotypes range between 2.3 and 2.4 Mb. The restriction pattern profiles of AscI, ApaI and NheI digested chromosomes showed a high degree of polymorphism among the different serotype reference strains and allowed their discrimination. The analysis of the macrorestriction pattern polymorphism revealed phylogenetic relationships between the different serotype reference strains ...
Subjects
free text keywords: Genetics, Molecular Biology, Microbiology, 630 Agriculture, Pulsed-field gel electrophoresis, Serotype, Genome size, Gel electrophoresis, Phylogenetic tree, Chromosome, Genome, Biology, Actinobacillus pleuropneumoniae, biology.organism_classification
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