publication . Doctoral thesis . 2015

Optimization of Glucose oxidase towards oxygen independency and high mediator activity for amperometric glucose determination in diabetes analytics

Arango Gutierrez, Erik Uwe;
Open Access English
  • Published: 01 Jan 2015
  • Publisher: Publikationsserver der RWTH Aachen University
  • Country: Germany
Abstract
Glucose oxidase is an oxidoreductase exhibiting a high β-D-glucose specificity and high stability which renders glucose oxidase well-suited for applications in diabetes care. Nevertheless, GOx activity is highly oxygen dependent which can lead to inaccuracies in amperometric β-D-glucose determinations. Therefore a directed evolution campaign with two rounds of random mutagenesis (SeSaM followed by epPCR), site saturation mutagenesis studies, and one simultaneous site saturation library (OmniChange; 4 positions) was performed in the first project part. A for diabetes care well suited mediator (quinone diimine) was selected and the GOx variant (T30V I94V) served a...
Subjects
free text keywords: Biowissenschaften, diabetes, Biologie, ddc:570
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