publication . Doctoral thesis . 2016

Akkumulation von L-Malat und D-Lactat in Arabidopsis thaliana und Laccase/HBT-vermittelte Delignifizierung von Spartina alterniflora und Phragmites australis

Heil, Alexander;
Open Access German
  • Published: 01 Jan 2016
  • Country: Germany
Abstract
The current work contains two projects "Accumulation of L-malate and D-lactate in Arabidopsis thaliana" (A) "Laccase/HBT mediated delignification of Spartina alterniflora and Phragmites australis" (B). In project A, L-malate and D-lactate accumulated in A. thaliana plants. The accumulation of L-malate is carried out by modification of the plant metabolism with the enzymes PEPC, MDH and the tonoplast dicarboxylate transporter (TDT). Gene pepci2 (Hydrilla verticillata), mdh5 (Zea mays) and tdt (Arabidopsis thaliana) were cloned in a plant expression vector pTRA-K-PEPCi2-TDT-$\lambda$F-MDH5. These genes were combined and stable transformed into A. thaliana plants. ...
Subjects
Medical Subject Headings: food and beveragesfungi
free text keywords: Laccase, Lactat, Malat, MDH, PEPC, TDT, ddc:570
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