Advances in directed monooxygenase evolution : from diversity generation and flow cytometry screening to tailor-made monooxygenases

Doctoral thesis English OPEN
Ruff, Anna Joëlle;
(2012)
  • Publisher: Publikationsserver der RWTH Aachen University
  • Subject: Ortspezifische Mutagenese | Gerichtete Evolution | Mutagenese | Durchflusscytometrie | Fluoreszenzaktivierter Zellsortierer | Proteindesign | Cytochrom P-450 | Monooxygenasen | Biowissenschaften, Biologie | In-vitro-Evolution | gelenkte Evolution | P450 BM3 | Vielfaltsgenerierung | Mutanten-Bibliotheken | directed evolution | random mutagenesis | flow cytometrie
    • ddc: ddc:570

Directed Evolution became a powerful tool for proteins engineers to generate tailor-made biocatalyst. Directed protein evolution consist of the following three consecutive main steps, which are performed in iterative cycles; Step 1 the gene diversity generation, Step 2 ... View more
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