publication . Doctoral thesis . 2012

A fundamental research of growth, metabolism and product formation of tobacco suspension cells at different scales

Ullisch, David;
Open Access English
  • Published: 01 Jan 2012
  • Publisher: Publikationsserver der RWTH Aachen University
  • Country: Germany
For over two decades, plant cell cultures have been promising hosts for the expression of recombinant proteins such as hormones, growth factors, full-size antibodies and antigens. So far, over 700 different plant cell cultures are stored in the German Collection of Microorganisms and Cell Cultures (DSMZ) in Braunschweig. Among these plant cell cultures, the tobacco cell line Nicotiana tabacum Bright Yellow 2 (BY-2) was chosen as a good host cell line for the production of recombinant proteins, as this cell line is well characterized – showing high growth rates and high cell synchrony. Up to now, individual studies have only handled one or two parameters (i.e. bi...
free text keywords: Tabak, Zellkultur, Biowissenschaften, Biologie, Nicotiana tabacum, Medienoptimierung, RAMOS, Sauerstofftransfer, Schüttelkolben, nicotiana tabacum, media optimization, oxygen transfer rate, shake flasks, ddc:570
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