Diseño asistido por computadora de la inmovilización covalente de bromelina y papaína

Article English OPEN
Cutiño-Avila, Bessy; Gil Pradas, Dayrom; Aragón Abreu, Carlos; Fernández Marrero, Yuniel; Hernández de la Torre, Martha; Salas Sarduy, Emir; Chávez Planes, María de los Ángeles; Guisán Seijas, José Manuel; Díaz Brito, Joaquín; del Monte-Martínez, Alberto;
  • Publisher: Instituto de Biotecnología, Universidad Nacional de Colombia
  • Subject: rational design | diseño racional | derivados inmovilizados | papaína | covalent immobilization | immobilized derivatives | bromelina | inmovilización covalente | papain | bromelain

Enzymes as immobilized derivatives have been widely used in Food, Agrochemical, Pharmaceutical and Biotechnological industries. Protein immobilization is probably the most used technology to improve the operational stability of these molecules. Bromelain (Ananas comosus... View more
  • References (31)
    31 references, page 1 of 4

    Abbenante, G., Fairlie, D.P. 2005. Protease Inhibitors in the Clinic. Medicinal Chemistry. 1: 71-104.

    Anvar, A., Saleemuddin, M. 2002. Purification and characterization of digestive alkaline protease from the larvae of Spilosoma obliqua. Archiv. Insect Biochem. Physiol. 51: 1-12.

    Bradford, M.M. 1976. A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72: 248-254.

    Cutiño-Avila, B., Cunill-Semanat, E., Gil, D.F., Chávez, M.A., Díaz, J., del Monte-Martínez, A. 2013. Synthesis of Tetanus ToxoidSepharose CL 4B derivatives by Rational Design. In: Ruiz Ll., Susana B. V Latin American Congress on Biomedical Engineering CLAIB 2011, IFMBE Proceedings 33. 800. ISBN 978-3-642- 21197-3.

    del Monte, A., Nolasco, H., Forrellat, A., Aragón, C., García, A., Díaz, J., Carrillo, O. 2002. Evidencias de la presencia de lipasas en el hepatopáncreas de Litopenaeus schmitii. CIVA 2002 (http:// www.civa2002.org), AquaTIC. 6: 207-222.

    del Monte-Martínez, A., Cutiño-Avila, B. 2012. Rational design of immobilized lipases and phospholipases. J. Meth. Mol. Biol. 861(4): 343-82.

    Delfín, J., Morera, V., González, Y., Díaz, J., Márquez, M., Larionova, N., Saroyán, A., Padrón, G., Chávez, M. 1996. Purification, characterization and immobilization of proteinase inhibitors from Stichodactyla helianthus. Toxicon. 34: 1367- 1376.

    Dunn, B. 1989. Protease mechanism. In: Beynon, R., Bond J. Proteolytic Enzymes. A Practical Approach. Oxford University Press, Oxford. 57-59.

    Guisán, J.M. 1988. Aldehyde-agarose gels as activated supports for immobilisation-stabilization of enzymes. Enzyme and Microbrial Technology. 10: 375-382.

    Guisán, J.M., Fernández-Lafuente, R., Rodríguez, V., Bastida, A., Blanco, R.M., Alvaro, G. 1993. Enzyme stabilization by multipoint covalent attachment to activated pre-existing supports. In: Van del Tweel W.J.J., Harder A., Buitelaar R.M., editors. Stability Stabilization of Enzymes. Amsterdam: Elsevier Science Publisher B.V. 55-62.

  • Similar Research Results (2)
  • Bioentities (1)
    9pap Protein Data Bank
  • Metrics
Share - Bookmark