Protección preferencial de los dominios II y III de la toxina Cry1Aa de Bacillus thuringiensis en Vesículas de Membrana de Borde de Cepillo

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Syed-Rehan A., Hussain; Flórez, Álvaro M.; Donald H, Dean; Alzate, Óscar;
(2010)
  • Publisher: Instituto de Biotecnología, Universidad Nacional de Colombia
  • Subject: δendotoxina | δ -endotoxin | site directed mutagenesis | mutagénesis sitio dirigida | Bacillus thuringiensis
    mesheuropmc: fungi

The surface exposed Leucine 371 on loop 2 of domain II, in Cry1Aa toxin, was mutated to Lysine to generate the trypsin-sensitive mutant, L371K. Upon trypsin digestion L371K is cleaved into approximately 37 and 26 kDa fragments. These are separable on SDS-PAGE, but remai... View more
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