publication . Article . 2017

Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile

PEREIRA, GUILLERMO; ALBORNOZ, VERÓNICA; ROMERO, CHRISTIAN; LARA, SEBASTIÁN; SÁNCHEZ-OLATE, MANUEL; RÍOS, DARCY; ATALA, CRISTIAN;
Open Access English
  • Published: 01 Jun 2017
  • Publisher: Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
Abstract
ABSTRACT Orchids require symbiotic fungi and/or specific conditions to germinate. Asymbiotic techniques have been shown successful for orchid germination. In Chile, Chloraea include many endemic, and potentially ornamental, terrestrial orchid species. In this study, individuals of Chloraea crispa, C. gavilu and C. virescens were manually autopollinated. The resulting capsules were sterilized and seeds were aseptically obtained. We evaluated asymbiotic germination in: Agar Water (AW), Knudson C (KC), Banana Culture Media (CMB), Tomato Culture Media (CMT), Malmgren Modified (MM), Murashige and Skoog (MS), and MS modified (MS1/2). Seeds were incubated in the dark a...
Subjects
free text keywords: Terrestrial orchids, endemic species, in vitro germination, culture media, orchid propagation, asymbiotic germination, Orquídeas terrestres, especies endémicas, germinación in vitro, medios de cultivo, propagación de orquídeas, germinación asimbiótica
53 references, page 1 of 4

ABRAHAM, S., AUGUSTINE, J., THOMAS, T.D. 2012. Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A. Rhich. an endemic orchid to Western Ghats. Physiology and Molecular Biology of Plants 18(2): 245- 251.

AGGARWAL, S., ZETTLER, L.W. 2010. Reintroduction of an endangered terrestrial orchid Dactylorhiza hatagirea (D. Don) Soo assisted by symbiotic seed germination: first report from the Indian subcontinent. Nature and Science 8(10): 139-145.

ARDITTI, J., CLEMENTS, M.A., FAST, G., HADLEY, G., NISHIMURA, G., & ERNST, R. 1982. Orchid seed germination and seedling culture. A manual. In: J. Arditti (ed.), Orchid biology. Reviews and perspectives II, pp. 243-370. Cornell University Press, Ithacha, NY, USA.

ARDITTI, J., ERNST, R., YAM, T.W., GLABE, C. 1990. A contribution of orchid mycorrhizal fungi to seed germination: a speculative review. Lindleyana 5: 249-255.

ATALA, C., MUÑOZ-TAPIA, L., PEREIRA, G., ROMERO, C., VARGAS, R., ACUÑA-RODRÍGUEZ, I.S., MOLINA-MONTENEGRO, M.A., BRITO, E. 2017. The effect of future climate change on the conservation of Chloraea disoides Lindl. (Orchidaceae) in Chile. Brazilian Journal of Botany 40(1): 353-360.

ATALA, C., PEREIRA, G., ROMERO, C., MUÑOZ-TAPIA, L., VARGAS, R., SUZ, L.M. 2015. Orchidiod fungi of the form-genus Rhizoctonia associated with the roots of Chloraea cuneata Lindl. From Araucanía, Chile. Gayana Botánica 72(1): 145-148. [OpenAIRE]

BARBERY, R., MOLARES, I. 2011. Manual para el cultivo in vitro de la orquídea Cattleya nobilior. Flor símbolo de Concepción. CEPAD, Editorial El País, Santa Cruz. 46 pp.

BERNHARDT, P. 1995. Biogeography and floral evolution in the Geoblasteae (Orchidaceae). In: M.T.K. Arroyo, P.H. Zedler & M.D. Fox (eds.), Ecology and biogeography of mediterranean ecosystems in Chile, California, and Australia, pp 116-134. Springer, Berlin, Germany.

CORREA, M.N. 1969. Chloraea, género sudamericano de Orchidaceae. Darwiniana 15: 374-500.

DEARNALEY, J.D. 2007. Further advances in orchid mycorrhizal research. Mycorrhiza17: 475-486. [OpenAIRE]

DEARNALEY, J.D., MARTOS, F., SELOSSE, M.A. 2012. Orchid mycorrhizas: molecular ecology, physiology, evolution, and conservation aspects. In: B. Hock (ed.), Fulgal associations, 2nd edn., pp. 207-230. Springer-Verlag, Berlin, Germany. [OpenAIRE]

DIXON, K.W. 1991. Seeder/clonal concepts in Western Australian orchids. In: T.C.E. Wells & J.H. Willems. (eds.), Population Ecology of Terrestrial Orchids, pp. 111-123. SPB Academic Publishing, The Hague, The Netherlands.

DUTRA, D., JOHNSON, T., KAUTH, P., STEWART, S., KANE, M., RICHARDSON, L. 2008. Asymbiotic seed germination, in vitro seedling development, and greenhouse acclimatization of the threatened terrestrial orchid Bletia purpurea. Plant Cell, Tissue and Organ Culture 94: 11-21.

ELÓRTEGUI, S., NOVOA, P. 2009. Orquídeas de la Región de Valparaíso. Taller la Era, Viña del Mar. 83 pp.

FRACCHIA, S., ARANDA-RICKERT, A., FLACHSLAND, E., TERADA, G., SEDE, S. 2014. Mycorrhizal compatibility and symbiotic reproduction of Gavilea australis, an endangered terrestrial orchid from south Patagonia. Mycorrhiza 24: 627-634.

53 references, page 1 of 4
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publication . Article . 2017

Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile

PEREIRA, GUILLERMO; ALBORNOZ, VERÓNICA; ROMERO, CHRISTIAN; LARA, SEBASTIÁN; SÁNCHEZ-OLATE, MANUEL; RÍOS, DARCY; ATALA, CRISTIAN;