Aldefluor protocol to sort keratinocytes stem cells from skin

Article English OPEN
Noronha,Samuel Marcos Ribeiro; Gragnani,Alfredo; Pereira,Thiago Antônio Calado; Correa,Silvana Aparecida Alves; Bonucci,Jessica; Ferreira,Lydia Masako;
(2017)
  • Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
  • Identifiers: doi: 10.1590/s0102-865020170110000010
  • Subject: Flow Cytometry | Keratinocytes | Adult Stem Cells.

Abstract Purpose: To investigate the use Aldefluor® and N, N - Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor® aliquots were prepared and maintained ... View more