publication . Article . 2012

5-Lipoxygenase contributes to PPAR [gamma] activation in macrophages in response to apoptotic cells

Knethen, Andreas von; Eifler, Lisa; Kuchler, Laura; Heeg, Annika; Heide, Heinrich; Wittig, Ilka; Maier, Thorsten Jürgen; Steinhilber, Dieter; Brüne, Bernhard;
Open Access English
  • Published: 14 Nov 2012
  • Country: Germany
Abstract
Background: One hallmark contributing to immune suppression during the late phase of sepsis is macrophage polarization to an anti-inflammatory phenotype upon contact with apoptotic cells (AC). Taking the important role of the nuclear receptor PPARγ for this phenotype switch into consideration, it remains elusive how AC activate PPARγ in macrophages. Therefore, we were interested to characterize the underlying principle. Methods: Apoptosis was induced by treatment of Jurkat T cells for 3 hours with 0.5 μg/ml staurosporine. Necrotic cells (NC) were prepared by heating cells for 20 minutes to 65°C. PPARγ activation was followed by stably transducing RAW264.7 macrop...
Subjects
Medical Subject Headings: lipids (amino acids, peptides, and proteins)
free text keywords: ddc:570, ddc:610
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