publication . Doctoral thesis . 2003

Untersuchungen zum Einfluss einer Air-Liquid-Interphase auf die Differenzierung von Alveolarepithelzellen Typ II

Sinis, Nektarios;
Open Access German
  • Published: 01 Jan 2003
  • Publisher: Justus-Liebig-Universität Gießen
  • Country: Germany
Abstract
Die Dedifferenzierung der Alveolarepithelzellen Typ II stellt in vitro ein großes Problem dar. Zellen, die länger als 48 Stunden in vitro kultiviert werden tendieren zu einer Dedifferenzierung. <br>Die vorliegende Arbeit stellt ein Air-Liquid-Interphasesystem zur Kultivierung von TypII-Zellen vor. Hierdurch ist es uns gelungen TypII-Zellen über einen Zeitraum von bis zu 14 Tagen in vitro auf einem hohen Grad der Differenzierung zu konservieren. Zellen die nicht so kultiviert wurden zeigten früh (48 Stunden) eine Dedifferenzierung. Untersucht wurden die Morphologie, metabolische Eigenschaften (Surfactanthaushalt), inflammatorische Faktoren und der Einfluß von Hyp...
Subjects
free text keywords: Alveolarepithelzellen Typ II, Air-Liquid-Interphase, Differenzierung, Surfactant, Hypoxie, Type II alveolar epithelial cells, Differentiation, Hypoxia, Medical sciences Medicine, ddc:610
Related Organizations
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