publication . Doctoral thesis . 1983

Cytosolic cholesterol ester hydrolase in adrenal cortex

Tocher, Douglas R.;
Open Access
  • Published: 01 Jan 1983
  • Publisher: The University of Edinburgh
  • Country: United Kingdom
Abstract
Cholesterol ester hydrolase (CEH) in adrenocortical cytosol was known to be phosphorylated and activated, in response to ACTH in a cAMPdependent protein kinase mediated process. The purification of CEH from bovine adrenocortical cytosol was attempted. The use of detergents to solubilise the enzyme from lipid-rich aggregates was investigated and sodium cholate was found to be effective. A purification procedure using cholate solubilised enzyme was developed. The detergent interfered in the operation of the ion-exchange columns, and pure enzyme was not obtained. Hydrophobic chromatography was studied but it was found to be unsuitable for the purification of CEH un...
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free text keywords: Annexe Thesis Digitisation Project 2016 Block 5
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