publication . Doctoral thesis . 2016

The mechanism of recognition and processing of DNA damage and modifications by RNA polymerase II

Shin, JI;
Open Access English
  • Published: 01 Jan 2016
  • Publisher: eScholarship, University of California
  • Country: United States
Abstract
RNA polymerase II (pol II) recognizes many obstacles during transcription elongation, including DNA damage lesions and modifications, via specific interactions and leads to distinct transcriptional outcomes. We investigate three specific types of modifications/lesions in DNA and how they affect the pol II transcription process: 1) unnatural synthetic nucleotides (dNaM and dTPT3), 2) regioisomeric alkylated thymidine lesions (O2-, N3-, O4-EtdT), and 3) non-covalent minor groove DNA binders pyrrole-imidazole (Py-Im) polyamides. In Chapter 1, we investigate pol II transcription and elongation in the presence of synthetic nucleotides (dNaM and dTPT3), and the abilit...
Subjects
free text keywords: Biology, Biochemistry, DNA damage, DNA modifications, RNA polymerase II, transcription
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