publication . Conference object . 2003

Control of single-molecule fluorescence dynamics by stimulated emission depletion

Richard J. Marsh; Mark A. Osborne; Angus J. Bain;
Open Access English
  • Published: 20 Nov 2003
  • Publisher: Society of Photo-Optical Instrumentation Engineers (SPIE)
  • Country: United Kingdom
Abstract
The feasibility of manipulating the single molecule absorption-emission cycle using picosecond stimulated emission depletion (STED) is investigated using a stochastic computer simulation. In the simulation the molecule is subjected to repeated excitation and depletion events using time delayed pairs of excitation (PUMP) and depletion (DUMP) pulses derived from a high repetition rate pulsed laser system. The model is used to demonstrate that a significant and even substantial reduction in the occurrence of 'dark states' in the fluorescence emission can be achieved using stimulated emission depletion. Variation in the PUMP-DUMP window allows precise control of the...
Subjects
free text keywords: Laser, law.invention, law, Analytical chemistry, Chemistry, Single-molecule experiment, Luminescence, Molecular physics, Fluorescence, Stimulated emission, Picosecond, Excitation, STED microscopy
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publication . Conference object . 2003

Control of single-molecule fluorescence dynamics by stimulated emission depletion

Richard J. Marsh; Mark A. Osborne; Angus J. Bain;