publication . Article . 2009

Rat Monoclonal Antibodies Specific for LST1 Proteins

Christian B. Schiller; Maximilian J.E. Nitschké; Alexander Seidl; Elisabeth Kremmer; Elisabeth H. Weiss;
Open Access English
  • Published: 10 Aug 2009
  • Country: Germany
Abstract
The LST1 gene is located in the human MHC class III region and encodes transmembrane and soluble isoforms that have been suggested to play a role in the regulation of the immune response and are associated with inflammatory diseases such as rheumatoid arthritis. Here we describe the generation and characterization of the first monoclonal antibodies against LST1. Two hybridoma lines secreting monoclonal antibodies designated 7E2 and 8D12 were established. The 7E2 antibody detects recombinant and endogenous LST1 by Western blot analysis while 8D12 reacts with recombinant and endogenous LST1 in immunoprecipitation and flow cytometry procedures. The newly establishe...
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free text keywords: Biologie, Immunology, Immunology and Allergy, ddc:570, MHC class I, biology.protein, biology, Recombinant DNA, law.invention, law, Antibody, Monoclonal antibody, medicine.drug_class, medicine, Western blot, medicine.diagnostic_test, Molecular biology, Immunoprecipitation, Transmembrane protein, Gene isoform
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2. Tatiana Nedelko, Heike Kollmus, Frank Klawonn, Sabine Spijker, Lu Lu, Manuela Heßman, Rudi Alberts, Robert W Williams, Klaus Schughart. 2012. Distinct gene loci control the host response to influenza H1N1 virus infection in a time-dependent manner. BMC Genomics 13:1, 411. [CrossRef]

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