publication . Doctoral thesis . 2004

Genetic diversity and molecular characterization of Saccharomyces cerevisiae strains from winemaking environments

Schuller, Dorit Elisabeth;
Open Access English
  • Published: 01 Jan 2004
  • Country: Brazil
Abstract
Tese de doutoramento em Ciências The principal aim of the present work is to assess the genetic diversity of fermenting Saccharomyces cerevisiae strains found in vineyards belonging to the Vinho Verde Region in order to create a strain collection representing the region’s biodiversity wealth as a basis for future strain selection and improvement programs. Validation of molecular techniques for accurate genotyping is an indispensable prerequisite for biogeographical surveys. Molecular typing methods (microsatellite analysis, restriction fragment length polymorphism of mitochondrial DNA (mtDNA RFLP), an optimized PCR-based interdelta method and electrophoretic kar...
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