publication . Article . 2017

Cell sheet engineering using the stromal vascular fraction of adipose tissue as a vascularization strategy

M. Costa; Mariana T. Cerqueira; T. C. Santos; Belém Sampaio-Marques; Paula Ludovico; Alexandra P. Marques; Rogério P. Pirraco; Rui L. Reis;
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  • Published: 24 Mar 2017
  • Publisher: Elsevier Ltd.
  • Country: Portugal
Abstract
Current vascularization strategies for Tissue Engineering constructs, in particular cell sheet-based, are limited by time-consuming and expensive endothelial cell isolation and/or by the complexity of using extrinsic growth factors. Herein, we propose an alternative strategy using angiogenic cell sheets (CS) obtained from the stromal vascular fraction (SVF) of adipose tissue that can be incorporated into more complex constructs. Cells from the SVF were cultured in normoxic and hypoxic conditions for up to 8 days in the absence of extrinsic growth factors. Immunocytochemistry against CD31 and CD146 revealed spontaneous organization in capillary-like structures, m...
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free text keywords: Cell sheet engineering, Stromal vascular fraction, Vascularization, Adipose tissue, SVF, Hypoxia, Science & Technology, Molecular Biology, Biomedical Engineering, Biochemistry, Biomaterials, General Medicine, Biotechnology, Stromal vascular fraction, Pathology, medicine.medical_specialty, medicine, Biology, Cell sheet, European research, Adipose tissue, Immunology
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