publication . Article . 2017

Applying phasor approach analysis of multiphoton FLIM measurements to probe the metabolic activity of three-dimensional in vitro cell culture models

Lakner, Pirmin H.; Monaghan, Michael G.; Möller, Yvonne; Olayioye, Monilola A.; Schenke-Layland, Katja;
Open Access
  • Published: 13 Feb 2017 Journal: Scientific Reports, volume 7 (eissn: 2045-2322, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
  • Country: Germany
Abstract
Fluorescence lifetime imaging microscopy (FLIM) can measure and discriminate endogenous fluorophores present in biological samples. This study seeks to identify FLIM as a suitable method to non-invasively detect a shift in cellular metabolic activity towards glycolysis or oxidative phosphorylation in 3D Caco-2 models of colorectal carcinoma. These models were treated with potassium cyanide or hydrogen peroxide as controls, and epidermal growth factor (EGF) as a physiologically-relevant influencer of cell metabolic behaviour. Autofluorescence, attributed to nicotinamide adenine dinucleotide (NADH), was induced by two-photon laser excitation and its lifetime decay...
Subjects
free text keywords: Multidisciplinary, Epidermal growth factor, Glycolysis, Oxidative phosphorylation, Nicotinamide adenine dinucleotide, chemistry.chemical_compound, chemistry, Hydrogen peroxide, Biophysics, Potassium cyanide, Fluorescence-lifetime imaging microscopy, Autofluorescence, Biology, Article
Funded by
EC| MIR-OPTOFECTARRAY
Project
MIR-OPTOFECTARRAY
Delivering non-viral silencing RNA (microRNA) using automated femtosecond lasers into stem cells for cardiac reprogramming and characterization using non-destructive optical techniques
  • Funder: European Commission (EC)
  • Project Code: 331430
  • Funding stream: FP7 | SP3 | PEOPLE
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