publication . Article . 2015

Characterization of Iranian nonaflatoxigenic strains of Aspergillus flavus based on microsatellite-primed PCR

Houshyarfard, Mahmoud; Rouhani, Hamid; Falahati-Rastegar, Mahrokh; Malekzadeh-Shafaroudi, Saeid; Mahdikhani-Moghaddam, Esmat;
Open Access English
  • Published: 01 Jan 2015 Journal: Molecular Biology Research Communications, volume 4, issue 1, pages 43-55 (issn: 2322-181X, eissn: 2345-2005, Copyright policy)
  • Publisher: Shiraz University
Abstract
Out of fifty-two Iranian nonaflatoxigenic strains of Aspergillus flavus,collected from various substrates (soil and kernel) and sources (peanut, corn and pistachio), fifteen representatives were selected according to their different geographical origins (six provinces: Guilan and Golestan, Ardebil, Fars, Kerman and Semnan) and vegetative compatibility groups (VCGs, IR1 to IR15) for microsatellite-primed PCR analysis. Two inter-simple sequence repeat (ISSR) primers AFMPP and AFM13 were used to determine polymorphism and the relationship among strain isolates. A. flavus isolates were identified by their morphologies and their identities were confirmed by PCR ampli...
Subjects
Medical Subject Headings: food and beverages
free text keywords: Original Article, Aflatoxin, Molecular marker, Inter-simple sequence repeat, Polymorphism
Related Organizations
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