publication . Other literature type . Article . 2011

Heterogeneous accumulation of fluorescent bile acids in primary rat hepatocytes does not correlate with their homogenous expression of ntcp

Pijun Wang; Amar Thosani; Allan W. Wolkoff; John W. Murray;
Open Access English
  • Published: 07 Apr 2011
  • Publisher: American Physiological Society
Abstract
<jats:p> Sodium taurocholate-cotransporting polypeptide (ntcp) is considered to be a major determinant of bile acid uptake into hepatocytes. However, the regulation of ntcp and the degree that it participates in the accumulation of specific substrates are not well understood. We utilized fluorescent bile acid derivatives and direct quantitation of fluorescent microscopy images to examine the regulation of ntcp and its role in the cell-to-cell variability of fluorescent bile acid accumulation. Primary-cultured rat hepatocytes rapidly accumulated the fluorescent bile acids, chenodeoxycholylglycylamidofluorescein (CDCGamF), 7-β- nitrobenzoxadiazole 3-α hydroxy 5-β ...
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Medical Subject Headings: digestive system
free text keywords: Liver and Biliary Tract, Hepatology, Gastroenterology, Physiology (medical), Physiology, Organic anion-transporting polypeptide, biology.protein, biology, Transfection, Bile acid, medicine.drug_class, medicine, HeLa, biology.organism_classification, Membrane permeability, Organic anion transporter 1, Fluorescence microscope, Biochemistry, Viability assay
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