publication . Article . Other literature type . 2011

Heterogeneous accumulation of fluorescent bile acids in primary rat hepatocytes does not correlate with their homogenous expression of ntcp.

John W Murray; Amar J Thosani; Pijun Wang; Allan W Wolkoff;
Open Access
  • Published: 07 Apr 2011 Journal: American Journal of Physiology-Gastrointestinal and Liver Physiology, volume 301, pages G60-G68 (issn: 0193-1857, eissn: 1522-1547, Copyright policy)
  • Publisher: American Physiological Society
Abstract
<jats:p> Sodium taurocholate-cotransporting polypeptide (ntcp) is considered to be a major determinant of bile acid uptake into hepatocytes. However, the regulation of ntcp and the degree that it participates in the accumulation of specific substrates are not well understood. We utilized fluorescent bile acid derivatives and direct quantitation of fluorescent microscopy images to examine the regulation of ntcp and its role in the cell-to-cell variability of fluorescent bile acid accumulation. Primary-cultured rat hepatocytes rapidly accumulated the fluorescent bile acids, chenodeoxycholylglycylamidofluorescein (CDCGamF), 7-β- nitrobenzoxadiazole 3-α hydroxy 5-β ...
Subjects
Medical Subject Headings: digestive system
free text keywords: Hepatology, Gastroenterology, Physiology (medical), Physiology, HeLa, biology.organism_classification, biology, Transfection, Biochemistry, Organic anion-transporting polypeptide, biology.protein, Viability assay, Fluorescence microscope, Organic anion transporter 1, Membrane permeability, Bile acid, medicine.drug_class, medicine, Liver and Biliary Tract
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