publication . Other literature type . Article . 2018

Proteasome substrate capture and gate opening by the accessory factor PafE from Mycobacterium tuberculosis

Kuan Hu; Jordan B. Jastrab; Susan Zhang; Amanda Kovach; Gongpu Zhao; K. Heran Darwin; Huilin Li;
Open Access English
  • Published: 05 Feb 2018
  • Publisher: American Society for Biochemistry and Molecular Biology
Abstract
In all domains of life, proteasomes are gated, chambered proteases that require opening by activators to facilitate protein degradation. Twelve proteasome accessory factor E (PafE) monomers assemble into a single dodecameric ring that promotes proteolysis required for the full virulence of the human bacterial pathogen Mycobacterium tuberculosis. Whereas the best characterized proteasome activators use ATP to deliver proteins into a proteasome, PafE does not require ATP. Here, to unravel the mechanism of PafE-mediated protein targeting and proteasome activation, we studied the interactions of PafE with native substrates, including a newly identified proteasome su...
Subjects
free text keywords: Protein Synthesis and Degradation, Cell Biology, Biochemistry, Molecular Biology
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