publication . Other literature type . Article . 2018

Proteasome substrate capture and gate opening by the accessory factor PafE from Mycobacterium tuberculosis

Kuan Hu; Jordan B. Jastrab; Susan Zhang; Amanda Kovach; Gongpu Zhao; K. Heran Darwin; Huilin Li;
Open Access English
  • Published: 05 Feb 2018
  • Publisher: American Society for Biochemistry and Molecular Biology
In all domains of life, proteasomes are gated, chambered proteases that require opening by activators to facilitate protein degradation. Twelve proteasome accessory factor E (PafE) monomers assemble into a single dodecameric ring that promotes proteolysis required for the full virulence of the human bacterial pathogen Mycobacterium tuberculosis. Whereas the best characterized proteasome activators use ATP to deliver proteins into a proteasome, PafE does not require ATP. Here, to unravel the mechanism of PafE-mediated protein targeting and proteasome activation, we studied the interactions of PafE with native substrates, including a newly identified proteasome su...
free text keywords: Protein Synthesis and Degradation, Cell Biology, Biochemistry, Molecular Biology
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue