publication . Article . Other literature type . 2015

Identification of Factors in Regulating a Protein Ubiquitination by Immunoprecipitation: a Case Study of TRF2 on Human REST4 Ubiquitination.

Ovando-Roche, Patrick; Cui, Wei;
Open Access English
  • Published: 05 Jul 2015
  • Publisher: Bio-protocol LLC
  • Country: United Kingdom
Abstract
Ubiquitination is the first step of the ubiquitin-proteasome pathway that regulates cells for their homeostatic functions and is an enzymatic, protein post-translational modification process in which ubiquitin is transferred to a target protein substrate by a set of three ubiquitin enzymes (Weissman et al., 2011; Bhattacharyya et al., 2014; Ristic et al., 2014). Given the importance of this process, it is plausible that ubiquitination is under strict control by many factors and that the regulatory machineries are protein-specific. An assay for the detection of a specific protein ubiquitination will enable us to examine whether a factor has a function to regulate...
Subjects
free text keywords: Cell biology, Ubiquitin, biology.protein, biology, Protein ubiquitination, Immunoprecipitation, Article
Related Organizations
Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue
publication . Article . Other literature type . 2015

Identification of Factors in Regulating a Protein Ubiquitination by Immunoprecipitation: a Case Study of TRF2 on Human REST4 Ubiquitination.

Ovando-Roche, Patrick; Cui, Wei;