publication . Article . Other literature type . 1995

properties of proton pumping in response to blue light and fusicoccin in guard cell protoplasts isolated from adaxial epidermis of vicia leaves

Goh, C. H.; Oku, T.; Shimazaki, Ki.;
Open Access
  • Published: 01 Sep 1995 Journal: Plant Physiology, volume 109, pages 187-194 (issn: 0032-0889, eissn: 1532-2548, Copyright policy)
  • Publisher: American Society of Plant Biologists (ASPB)
Abstract
Guard cell protoplasts (GCPs) were isolated from the adaxial epidermis of Vicia leaves. The properties of isolated adaxial GCPs (ad GCPs) were compared with those of abaxial GCPs (ab GCPs) with respect to H+-pumping activity. A saturating pulse of blue light (200 [mu]mol m-2 s-1, 30 s) induced H+ pumping in both ad GCPs and ab GCPs under red light. The maximum rate of blue-light-dependent H+ pumping was slightly higher in ad GCPs than in ab GCPs, but the magnitude of H+ pumping in ad GCPs was 68% of that in ab GCPs. H+ pumping was responsive to the second pulse, and the rate and magnitude of the pumping increased with the time between two pulses. The periods req...
Subjects
free text keywords: Plant Science, Genetics, Physiology, Guard cell, Proton, Biology, Proton transport, Fusicoccin, chemistry.chemical_compound, chemistry, Epidermis (botany), Vicia, biology.organism_classification, Biochemistry, Protoplast, Diaphragm pump, Botany, Research Article
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publication . Article . Other literature type . 1995

properties of proton pumping in response to blue light and fusicoccin in guard cell protoplasts isolated from adaxial epidermis of vicia leaves

Goh, C. H.; Oku, T.; Shimazaki, Ki.;