publication . Article . Other literature type . 2016

Differential Membrane Binding Mechanics of Synaptotagmin Isoforms Observed in Atomic Detail

Vermaas, Josh V.; Tajkhorshid, Emad;
Open Access
  • Published: 20 Dec 2016 Journal: Biochemistry, volume 56, pages 281-293 (issn: 0006-2960, eissn: 1520-4995, Copyright policy)
  • Publisher: American Chemical Society (ACS)
Abstract
Synaptotagmin (Syt) is a membrane-associated protein involved in vesicle fusion through the SNARE complex that is found throughout the human body in 17 different isoforms. These isoforms have two membrane-binding C2 domains, which sense Ca2+ and thereby promote anionic membrane binding and lead to vesicle fusion. Through molecular dynamics simulations using the HMMM acclerated bilayer model, we have investigated how small protein sequence changes in the Ca2+ binding loops of the C2 domains may give rise to the experimentally determined difference in binding kinetics between Syt-1 and Syt-7 isoforms. Syt-7 C2 domains are found to form more close contacts with ani...
Subjects
free text keywords: Biochemistry, Synaptotagmin 1, Umbrella sampling, SNARE complex, Membrane, SNAP25, Chemistry, Bilayer, Receptor–ligand kinetics, Vesicle fusion, Article
Abstract
Synaptotagmin (Syt) is a membrane-associated protein involved in vesicle fusion through the SNARE complex that is found throughout the human body in 17 different isoforms. These isoforms have two membrane-binding C2 domains, which sense Ca2+ and thereby promote anionic membrane binding and lead to vesicle fusion. Through molecular dynamics simulations using the HMMM acclerated bilayer model, we have investigated how small protein sequence changes in the Ca2+ binding loops of the C2 domains may give rise to the experimentally determined difference in binding kinetics between Syt-1 and Syt-7 isoforms. Syt-7 C2 domains are found to form more close contacts with ani...
Subjects
free text keywords: Biochemistry, Synaptotagmin 1, Umbrella sampling, SNARE complex, Membrane, SNAP25, Chemistry, Bilayer, Receptor–ligand kinetics, Vesicle fusion, Article
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publication . Article . Other literature type . 2016

Differential Membrane Binding Mechanics of Synaptotagmin Isoforms Observed in Atomic Detail

Vermaas, Josh V.; Tajkhorshid, Emad;