publication . Article . 2017

Cloning of canine Ku80 and its localization and accumulation at DNA damage sites

Koike, Manabu; Yutoku, Yasutomo; Koike, Aki;
Open Access
  • Published: 01 Nov 2017 Journal: FEBS Open Bio, volume 7, pages 1,854-1,863 (issn: 2211-5463, Copyright policy)
  • Publisher: Wiley
Abstract
Molecularly targeted therapies have high specificity and significant cancer‐killing effect. However, their antitumor effect might be greatly diminished by variation in even a single amino acid in the target site, as it occurs, for example, as a consequence of SNPs. Increasing evidence suggests that the DNA repair protein Ku80 is an attractive target molecule for the development of next‐generation radiosensitizers for human cancers. However, the localization, post‐translational modifications (PTMs), and complex formation of Ku80 have not been elucidated in canines. In this study, for the first time, we cloned, sequenced, and characterized canine Ku80 cDNA. Our da...
Subjects
free text keywords: Nuclear localization sequence, DNA Repair Protein, DNA damage, NLS, Complementary DNA, Ku70, Molecular biology, Ku80, DNA repair, Biology, Research Article, Research Articles, canine, companion animal, DNA double‐strand break, nonhomologous DNA‐end joining
32 references, page 1 of 3

1 Jacob JA (2016) Researchers turn to canine clinical trials to advance cancer therapies. JAMA 315, 1550–1552.27027696 [PubMed]

2 Paoloni M and Khanna C (2008) Translation of new cancer treatments from pet dogs to humans. Nat Rev Cancer 8, 147–156.18202698 [PubMed]

3 Lawrence J, Cameron D and Argyle D (2015) Species differences in tumour responses to cancer chemotherapy. Philos Trans R Soc Lond B Biol Sci 370, 20140233.26056373 [OpenAIRE] [PubMed]

4 Gavande NS, VanderVere‐Carozza PS, Hinshaw HD, Jalal SI, Sears CR, Pawelczak KS and Turchi JJ (2016) DNA repair targeted therapy: the past or future of cancer treatment? Pharmacol Ther 160, 65–83.26896565 [OpenAIRE] [PubMed]

5 Mahaney BL, Meek K and Lees‐Miller SP (2009) Repair of ionizing radiation‐induced DNA double‐strand breaks by non‐homologous end‐joining. Biochem J 417, 639–650.19133841 [OpenAIRE] [PubMed]

6 Koike M (2002) Dimerization, translocation and localization of Ku70 and Ku80 proteins. J Radiat Res (Tokyo) 43, 223–236.12518983 [OpenAIRE] [PubMed]

7 Koike M, Awaji T, Kataoka M, Tsujimoto G, Kartasova T, Koike A and Shiomi T (1999) Differential subcellular localization of DNA‐dependent protein kinase components Ku and DNA‐PKcs during mitosis. J Cell Sci 112, 4031–4039.10547363 [PubMed]

8 Koike M, Ikuta T, Miyasaka T and Shiomi T (1999) Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal. Oncogene 18, 7495–7505.10602508 [PubMed]

9 Koike M and Koike A (2008) Accumulation of Ku80 proteins at DNA double‐strand breaks in living cells. Exp Cell Res 314, 1061–1070.18164703 [OpenAIRE] [PubMed]

10 Wei S, Zhang ZY, Fu S, Xie JG, Liu XS, Xu YJ, Zhao JP and Xiong WN (2016) Hsa‐miR‐623 suppresses tumor progression in human lung adenocarcinoma. Cell Death Dis 7, e2388.27685632 [OpenAIRE] [PubMed]

11 Weterings E, Gallegos AC, Dominick LN, Cooke LS, Bartels TN, Vagner J, Matsunaga TO and Mahadevan D (2016) A novel small molecule inhibitor of the DNA repair protein Ku70/80. DNA Repair (Amst) 43, 98–106.27130816 [PubMed]

12 Koike M, Ikuta T, Miyasaka T and Shiomi T (1999) The nuclear localization signal of the human Ku70 is a variant bipartite type recognized by the two components of nuclear pore‐targeting complex. Exp Cell Res 250, 401–413.10413594 [PubMed]

13 Koike M, Yutoku Y and Koike A (2011) Establishment of Ku70‐deficient lung epithelial cell lines and their hypersensitivity to low‐dose X‐irradiation. J Vet Med Sci 73, 549–554.21160137 [OpenAIRE] [PubMed]

14 Koike M, Yutoku Y and Koike A (2016) Cloning, localization and focus formation at DNA damage sites of canine XRCC4. J Vet Med Sci 78, 1865–1871.27644316 [OpenAIRE] [PubMed]

15 Koike M, Yutoku Y and Koike A (2017) Cloning, localization and focus formation at DNA damage sites of canine Ku70. J Vet Med Sci 79, 554–561.28163277 [OpenAIRE] [PubMed]

32 references, page 1 of 3
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publication . Article . 2017

Cloning of canine Ku80 and its localization and accumulation at DNA damage sites

Koike, Manabu; Yutoku, Yasutomo; Koike, Aki;