publication . Other literature type . Article . 1995

The three genes lipB, lipC, and lipD involved in the extracellular secretion of the Serratia marcescens lipase which lacks an N-terminal signal peptide.

H Akatsuka; E Kawai; K Omori; T Shibatani;
Open Access English
  • Published: 01 Nov 1995
Abstract
The extracellular lipase of Serratia marcescens Sr41, lacking a typical N-terminal signal sequence, is secreted via a signal peptide-independent pathway. The 20-kb SacI DNA fragment which allowed the extracellular lipase secretion was cloned from S. marcescens by selection of a phenotype conferring the extracellular lipase activity on the Escherichia coli cells. The subcloned 6.5-kb EcoRV fragment was revealed to contain three open reading frames which are composed of 588, 443, and 437 amino acid residues constituting an operon (lipBCD). Comparisons of the deduced amino acid sequences of the lipB, lipC, and lipD genes with those of the Erwinia chrysanthemi prtDE...
Subjects
free text keywords: Research Article, Molecular Biology, Microbiology, Escherichia coli, medicine.disease_cause, medicine, ATP-binding cassette transporter, Peptide sequence, Serratia marcescens, biology.organism_classification, biology, Operon, Signal peptide, Extracellular, Lipase, biology.protein, Biochemistry
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publication . Other literature type . Article . 1995

The three genes lipB, lipC, and lipD involved in the extracellular secretion of the Serratia marcescens lipase which lacks an N-terminal signal peptide.

H Akatsuka; E Kawai; K Omori; T Shibatani;