Concentration-dependent exchange accelerates turnover of proteins bound to double-stranded DNA

Article English OPEN
Graham, John S.; Johnson, Reid C.; Marko, John F.; (2010)
  • Publisher: Oxford University Press
  • Journal: Nucleic Acids Research,volume 39,issue 6,pages2,249-2,259 (issn: 0305-1048, eissn: 1362-4962)
  • Related identifiers: pmc: PMC3064784, doi: 10.1093/nar/gkq1140
  • Subject: Molecular Biology

The multistep kinetics through which DNA-binding proteins bind their targets are heavily studied, but relatively little attention has been paid to proteins leaving the double helix. Using single-DNA stretching and fluorescence detection, we find that sequence-neutral DN... View more