publication . Other literature type . Article . 1984

Cloning and characterization of gdhA, the structural gene for glutamate dehydrogenase of Salmonella typhimurium.

E S Miller; J E Brenchley;
Open Access English
  • Published: 01 Jan 1984
Abstract Glutamic acid is synthesized in enteric bacteria by either glutamate dehydrogenase or by the coupled activities of glutamate synthase and glutamine synthetase. A hybrid plasmid containing a fragment of the Salmonella typhimurium chromosome cloned into pBR328 restores growth of glutamate auxotrophs of S. typhimurium and Escherichia coli strains which have mutations in the genes for glutamate dehydrogenase and glutamate synthase. A 2.2-kilobase pair region was shown by complementation analysis, enzyme activity measurements, and the maxicell protein synthesizing system to carry the entire glutamate dehydrogenase structural gene, gdhA. Glutamate dehydrogena...
free text keywords: Research Article, Molecular Biology, Microbiology, Structural gene, Glutamate synthase, biology.protein, biology, Glutamine synthetase, Plasmid, Biochemistry, Escherichia coli, medicine.disease_cause, medicine, Complementation, Glutamate dehydrogenase, Glutamic acid
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