publication . Other literature type . Article . 2014

HBV life cycle is restricted in mouse hepatocytes expressing human NTCP

Jun-Fang Zhang; Yali Zhang; Ningshao Xia; Quan Yuan; Yuze Wang; Yi Lin; Jiahuai Han; Jing-Hua Zhao; Qiuyu Zhuang; Tianying Zhang; ...
Open Access
  • Published: 10 Feb 2014
  • Publisher: Springer Science and Business Media LLC
Abstract
Recent studies have revealed that human sodium taurocholate cotransporting polypeptide (SLC10A1 or NTCP) is a functional cellular receptor for hepatitis B virus (HBV). However, whether human NTCP can support HBV infection in mouse hepatocyte cell lines has not been clarified. Because an HBV-permissible mouse model would be helpful for the study of HBV pathogenesis, it is necessary to investigate whether human NTCP supports the susceptibility of mouse hepatocyte cell lines to HBV. The results show that exogenous human NTCP expression can render non-susceptible HepG2 (human), Huh7 (human), Hepa1–6 (mouse), AML-12 (mouse) cell lines and primary mouse hepatocyte (PM...
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Subjects
Medical Subject Headings: digestive system diseasesvirus diseasesdigestive system
free text keywords: Research Article, Immunology, Immunology and Allergy, Infectious Diseases, Hepatitis B virus, medicine.disease_cause, medicine, Virology, SLC10A1, biology.protein, biology, Hepatitis D, medicine.disease, Virus genetics, Genetically modified mouse, Viral entry, Hepatitis B, Hepatitis D virus
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Abstract
Recent studies have revealed that human sodium taurocholate cotransporting polypeptide (SLC10A1 or NTCP) is a functional cellular receptor for hepatitis B virus (HBV). However, whether human NTCP can support HBV infection in mouse hepatocyte cell lines has not been clarified. Because an HBV-permissible mouse model would be helpful for the study of HBV pathogenesis, it is necessary to investigate whether human NTCP supports the susceptibility of mouse hepatocyte cell lines to HBV. The results show that exogenous human NTCP expression can render non-susceptible HepG2 (human), Huh7 (human), Hepa1–6 (mouse), AML-12 (mouse) cell lines and primary mouse hepatocyte (PM...
Persistent Identifiers
Subjects
Medical Subject Headings: digestive system diseasesvirus diseasesdigestive system
free text keywords: Research Article, Immunology, Immunology and Allergy, Infectious Diseases, Hepatitis B virus, medicine.disease_cause, medicine, Virology, SLC10A1, biology.protein, biology, Hepatitis D, medicine.disease, Virus genetics, Genetically modified mouse, Viral entry, Hepatitis B, Hepatitis D virus
Related Organizations
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