publication . Article . Other literature type . 2011

Adaptation of microplate-based respirometry for hippocampal slices and analysis of respiratory capacity.

Schuh, Rosemary A.; Clerc, Pascaline; Hwang, Hyehyun; Mehrabian, Zara; Bittman, Kevin; Chen, Hegang; Polster, Brian M.;
Open Access
  • Published: 21 Apr 2011 Journal: Journal of Neuroscience Research, volume 89, pages 1,979-1,988 (issn: 0360-4012, Copyright policy)
  • Publisher: Wiley
Abstract
Multiple neurodegenerative disorders are associated with altered mitochondrial bioenergetics. Although mitochondrial O2 consumption is frequently measured in isolated mitochondria, isolated synaptic nerve terminals (synaptosomes), or cultured cells, the absence of mature brain circuitry is a remaining limitation. Here we describe the development of a method that adapts the Seahorse Extracellular Flux Analyzer (XF24) for the microplate-based measurement of hippocampal slice O2 consumption. As a first evaluation of the technique, we compared whole slice bioenergetics to previous measurements made with synaptosomes or cultured neurons. We found that mitochondrial r...
Subjects
free text keywords: Cellular and Molecular Neuroscience, Bioenergetics, Mitochondrion, Cellular respiration, Respirometry, Biology, ATP synthase, biology.protein, Biochemistry, Oligomycin, chemistry.chemical_compound, chemistry, Respiration, Hippocampal formation, Article
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publication . Article . Other literature type . 2011

Adaptation of microplate-based respirometry for hippocampal slices and analysis of respiratory capacity.

Schuh, Rosemary A.; Clerc, Pascaline; Hwang, Hyehyun; Mehrabian, Zara; Bittman, Kevin; Chen, Hegang; Polster, Brian M.;