publication . Article . Other literature type . 2017

A novel single-cell method provides direct evidence of persistent DNA damage in senescent cells and aged mammalian tissues

Galbiati, Alessandro; Beauséjour, Christian; d'Adda di Fagagna, Fabrizio;
Open Access
  • Published: 01 Apr 2017 Journal: Aging Cell, volume 16, pages 422-427 (issn: 1474-9718, Copyright policy)
  • Publisher: Wiley
Abstract
Summary The DNA damage response (DDR) arrests cell cycle progression until DNA lesions, like DNA double-strand breaks (DSBs), are repaired. The presence of DSBs in cells is usually detected by indirect techniques that rely on the accumulation of proteins at DSBs, as part of the DDR. Such detection may be biased, as some factors and their modifications may not reflect physical DNA damage. The dependency on DDR markers of DSB detection tools has left questions unanswered. In particular, it is known that senescent cells display persistent DDR foci, that we and others have proposed to be persistent DSBs, resistant to endogenous DNA repair activities. Others have pro...
Subjects
Medical Subject Headings: body regions
free text keywords: Oligonucleotide, Molecular biology, Endogeny, Biology, DNA damage, DNA repair, DNA, chemistry.chemical_compound, chemistry, Genetics, Chromatin, Proximity ligation assay, Ligation, Short Take, aging, cellular senescence, DNA damage response, DNA damage in situ proximity ligation assay, DNA segments with chromatin alterations reinforcing senescence
Funded by
EC| DDRNA
Project
DDRNA
A novel direct role of non coding RNA in DNA damage response activation
  • Funder: European Commission (EC)
  • Project Code: 322726
  • Funding stream: FP7 | SP2 | ERC
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publication . Article . Other literature type . 2017

A novel single-cell method provides direct evidence of persistent DNA damage in senescent cells and aged mammalian tissues

Galbiati, Alessandro; Beauséjour, Christian; d'Adda di Fagagna, Fabrizio;