publication . Article . 2010

Role of endosomal membrane lipids and NPC2 in cholesterol transfer and membrane fusion[S]

Abdul-Hammed, Misbaudeen; Breiden, Bernadette; Adebayo, Matthew A.; Babalola, Jonathan O.; Schwarzmann, Günter; Sandhoff, Konrad;
Open Access English
  • Published: 01 Jan 2010 Journal: Journal of Lipid Research, volume 51, issue 7, pages 1,747-1,760 (issn: 0022-2275, eissn: 1539-7262, Copyright policy)
  • Publisher: The American Society for Biochemistry and Molecular Biology
Abstract
We examined the effect of Niemann-Pick disease type 2 (NPC2) protein and some late endosomal lipids [sphingomyelin, ceramide and bis(monoacylglycero)phosphate (BMP)] on cholesterol transfer and membrane fusion. Of all lipid-binding proteins tested, only NPC2 transferred cholesterol at a substantial rate, with no transfer of ceramide, GM3, galactosylceramide, sulfatide, phosphatidylethanolamine, or phosphatidylserine. Cholesterol transfer was greatly stimulated by BMP, little by ceramide, and strongly inhibited by sphingomyelin. Cholesterol and ceramide were also significantly transferred in the absence of protein. This spontaneous transfer of cholesterol was gre...
Subjects
Medical Subject Headings: lipids (amino acids, peptides, and proteins)
free text keywords: Research Articles, Niemann-Pick disease type 2 protein, ceramide, sphingomyelin, late endosomes, sphingolipid activator proteins
Funded by
EC| LIPIDOMICNET
Project
LIPIDOMICNET
Lipid droplets as dynamic organelles of fat deposition and release: Translational research towards human disease
  • Funder: European Commission (EC)
  • Project Code: 202272
  • Funding stream: FP7 | SP1 | HEALTH
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